Impact Of Chotrogenic Acid On Growth Performance, Blood Biochemical Indexes And Metallothionein Gene Expression Of Jiong Rabbit

Development and usage of substitutes of antibiotic supplements have beenreceived widespread attention in the light of the hazards brought by antibiotic. Thecurrent research focused on chlorogenic acid and investigated the feasibility ofchlorogenic acid being used as substitute of antibiotic. A total of8460-day-oldJirong rabbits were chosen in the experiment and were separated into seven groupsby the body weight. Group1was the blank control and fed with basic diet. Group2was antibiotic control and fed with basic diet supplemented with0.02%antibiotic.Group3to7were chlorogenic acid groups for which0.03%,0.09%,0.15%,0.21%and0.27%were supplemented in the basic diet. We tested the growth performance,slaughter performance and blood biochemical indices and studied the impact ofchlorogenic acid on digestibility of and metallothionein gene expression in liver ofJirong rabbit. Results: chlorogenic acid in diet improved growth performance ofJirong rabbit. Daily weight gain of0.03%-0.21%chlorogenic acid group wassignificantly higher than that of blank control and antibiotic group (P＜0.05). Chestcircumference of all chlorogenic acid groups was higher than that of blank controland antibiotic group though insignificantly (P＞0.05). Carcass weight of rabbits in0.03%chlorogenic acid group was significantly higher than that of antibiotic group(P＜0.05). There was no significant difference in slaughter rate and hides area(P>0.05). Fresh coat of0.03%-0.15%group was significantly larger than that ofblank control (P＜0.05), and fresh coat of0.03%and0.09%groups were bothlarger than antibiotic group,0.21%and0.27%group (P＜0.05). Supplementation ofchlorogenic acid significantly improved albumin content, lysozyme activity, SODactivity and metallothionein content (P＜0.05), but had insignificant influence onIgG (P＞0.05). Among others, crude protein, crude fat, crude ash component, crudefiber, neutral detergent fiber and acidic detergent fiber were all improved along withsupplementation of chlorogenic acid. And it was revealed that changes of blood biochemical indices and digestibility were associated with additional dose andeffect duration. Metallothionein gene expression in liver of Jirong rabbit with dietsupplemented with0.09%-0.21%was higher than that of blank control, antibioticgroup,0.03%and0.27%groups (P＜0.05). Therefore, chlorogenic acid can be usedas novel feed additive and an alternative in animal production and0.15%was thesuggested dose.