Introduction Of LTP、TR1into Soybean (Glycine Max (L.)Merr.) And Construction Of Twin T-DNA Binary Vector

Soybean (Glycine max (L.) Merr.) is an important oil, edible and fodder crop, and can be used as industrial material. As a new breeding method, genetic engineering has been applied to many crops. Diferent transformation methods in soybean were used, such as Agrobacterium-mediated gene transformation, particle bombardment, pollen-tube pathway, electroporation, PEG-mediated method, etc. and the former two are the most frequently used methods.In order to breed soybean varieties with resistances, LTP (lipid transfer protein) gene and TR1(thermal resistance1) gene were transferred to soybean by Agrobacterium-mediated cotyledonary node transformation method. Six and12positive plantlets were obtained with LTP and TR1gene, respectively. Inducement percentage of buds, plant regeneration percentage, and transformation percentage were73.74%,9.02%, and0.8%, respectively, for LTP-modified soybeans,58.31%,13.68%, and0.86%, respectively, for TR1-modified soybeans. Compared with previous reports, inducement percentage of buds was relatively high, but plant regeneration percentage and transformation percentage were relative low, so transformation system should be optimized further.In order to delete antibiotic gene used in the transformants, binary vector pSB130-TR1with two T-DNA regions were constructed to transform soybean, which laid a foundation for getting tolerance transgenic soybean without antibiotic makers.