Study On Effect Of Three Microbial Preparations And Their Combinations On Nutritive Value Of Micro-storage Rice Straw

There are three parts in this study:1. Microbial preparation of A,B,C and combinations were selected in this experiment for Micro-storage rice straw,and analysis by sensory evaluation and laboratory evaluation.2. By culture in vitro,gas production, NH3-N,BCP concentration of rice straw culture.3. By the nylon bag technique,rumen disappearance rate of DM,OM,NDF,ADF,CP of rice straw,in Jinjiang cattle.Microbial preparation of A,B,C and their combinations were selected in this experiment of Micro-storage rice straw.6treatment groups and control group was designed in the experiment. Experimental period is60days. Sensory quality and laboratory indexes were evaluated. The results showed as follow:(1) Sensory quality and palatability,of rice strew,were improved by microbial preparation and their combinations.(2)CP,EE and WSC content of rice straw in treatment groups were higher than that in control group (P<0.05). Content of DM,OM,NDF,ADF and NH3-N/TN rate of rice straw in different treatment groups were significantly decreased than that in control group (P<0.05).(3) Under this experimental condition and for micro-storage of rice straw. C group is best among single microbial preparation,and A+B+C is best among different combination groups,The results was provided evidence for popularization and application of rice straw in the practical productionThe second part,By culture in vitro,gas production,NH3-N,BCP concentration of rice straw culture,was Measured at6h,12h,24h,36h,48h,and that correlation of it was analysis. Four repelications in each time point of each treatment.The results showed that:(1) Gas production of treatments was higher than that in control group (P<0.05). Gas production of A group is maximum among single microbial preparation,and that of A+B group is maximum among different combinations.(2) NH3-N concentration of treatments was higher than that in control group (P<0.05),and that of every groups is suitable for the growth of the rumen microbes.(3) BCP concentration of treatments was higher than that in control group (P<0.05).(4) By comprehensive consideration concentration of NH3-N,BCP,A group is best among single microbial preparation,and A+B group is best among different combinations.(5) Correlation of concentration of BCP is middle to that of NH3-N. Regression equation of it is that: y=1.214x+10.232C Y: BCP concentration in culture liquid,X: NH3-N concentration in culture liquid,R2=0.6778)The third part,By the nylon bag technique,rumen disappearance rate of DM,OM, NDF,ADF,CP,of rice straw,in Jinjiang cattle,was Measured at6h,12h,24h,48h,72h.Three repelications in each time point.4g sample for each repelication,altogethger120nylon bags.The results showed that:(l)Rumen disappearance rate of DM,OM,NDF, ADF,CP of rice straw,in Jinjiang cattle,can be improved by adding different microbial preparation or combination.(2) Microbial preparation C is best among singles,the difference was significant compared with control (P<0.05),rumen disappearance rate of DM,OM,NDF,ADF,CP,of rice straw.(3) the difference of rumen disappearance rate of DM,OM,NDF,ADF,of rice straw,was not significant (P>0.05),between4microbial preparation combinations,the best is microbial preparation combination A+B; the difference of rumen disappearance rate of CP,of rice straw,of4microbial preparation combinations,was not significant (P>0.05) compared with control.(4) Microbial preparation combination is best for synthesis of microbial protein.