Breeding Fine Varieties Of Copyinds Comatus By Single Spore Isolation

This text use two mother materiales inside Copyinds comatus White36from Gaoyou edible mushroom graduate school from Jiangsu province and wild Copyinds comatus collect at Alar in Xinjiang province, study their biology characteristic, as to it’s carried on spores to collect and Single-spore to separate,after Single-spore hybridize hand over get lately hybridize varieties.And carry on to the hybridize varieties to choose excellent ones, expect to get high yield and good quality so as to accommodate with Alar region environment of the Copyinds comatus species.Show as a result under25℃conditions, especially the hypha long power, hypha density of White36germ stub attain the best level, the hypha color and luster is snow-White, and under30℃conditions the wild Copyinds comatus germ stub attains the best level.White36germ stub pH the value take7-8scope growths as good, but wild Copyinds comatus pH the value take6-7scope growths as good.The two kinds of Copyinds comatus germ stub hypha all can grow at glucose, fructose, malt sugar, starch, cane sugar five kinds of carbon source tops, but from the hypha growth speed of the difference see a glucose and fructose be iner keeping with growths of carbon source, explained the Copyinds comatus hypha to the single sugar of the exploitation want a ratio double sugar and many sugar to want quickly.Two kinds of Copyinds comatus germ stub hypha when the peptone, hamburger and zymogen make a nitrogen source, hypha of long soon show quick at the ammonium sulfate and ammonium nitrate, this expresses organic nitrogen utilization better than machine nitrogen.The spore collections experiments to express adopt the triangle bottle method collections ghost umbrella which refrigerates germ plait a spore of belong to the fungi, the pollution rate is low and to a large extent overcome again to up overcome a spore from dissolve of phenomenon, can at any time create a spore to suspend a liquid to carry on experiment, don’t need every time experiment all carry on spore collections.The spore of the Copyinds comatus germ stub develops under23℃～32℃conditions, all sprout and grow, especially White36sporeses sprout with grow the most suitable and proper temperature is26℃, wild Copyinds comatus spore’s sprouting and growing the most suitable and proper temperature is29℃.Especially White36sporeses sprout with grow the most suitable and proper pH is7.0, wild Copyinds comatus spore’s sprouting and growing the most suitable and proper t proper pH is7.5.Compare fructose for under the culture media condition of carbon source development condition, two kinds of spores of germ stubs are feater to sprout in take glucose as the culture media of carbon source with growth.Compare peptone and Yeast extract, two kinds of spores of germ stubs are feater to sprout in take beef extract as the culture media of nitrogen source with growth.Experiment to discover, the list pit hypha and double check a hypha to pack consociation, hypha in the pit piece, lock long power, the hypha be thick thin, hypha shape existence obvious difference.Experiment medium has been sprouted to two kinds of Copyinds comatus spores of the hypha carry on pick, dying, mirror check, each end acquire55body germ stubs.Miscellaneous handed over germ stub to authenticate to experiment enunciation and through20combinations don’t have lock form consociation,80combinations have the lock form consociation.Miscellaneous hand over combination with close origin carry on a Antagonistic experiment,23combines with close origin the hypha crosses mutually,then produced a new sex form,belong to same hypha,57have the anti-line.Miscellaneous hand over a combination to carry on a bottle to plant to experiment,9combines does’t produce mushroom and48combines produce mushroom.Eliminate don’t have burliness ability of miscellaneous hand over a combination, the end assurance acquired48miscellaneous hand over new germ stub.Plant a mushroom to experiment through a bottle miscellaneous to48stubs hand over new germ stub to carry on first step sieving, finally sieve to set out a germ period shorter, the growth of hypha is certainly stronger, sub-entity shape better chicken leg the mushroom be miscellaneous to hand over germ stub15stubs.Finally miscellaneous to15stubs hand over germ stub to carry on to choose further excellent experiment, pass respectively as to it’s hypha growth the measurement of the speed, growth period of the comparison, sub-entity agriculture learn the comparison, anti-of form liquefaction from dissolve of the comparison of comparison and yield, finally select miscellaneous hand over germ stub C11、C25and C47for miscellaneous hand over the advantage stronger germ stub, belong to good Copyinds comatus new germ stub.