Polymorphism Of NCOA1and ADAMTS1Genes Of Swine And Its Association With Reproductive Traits

NCOA1gene act mutually with Nuclear Receptor that bond to DNA and itstranscriptional activity is enhanced then. Androgen Receptor, estrogen receptor andreceptors that are influenced by feature in the physiological process of animal’s growth andbreeding. ADAMTS1which contain thrombin sensitive protein motif is a new foundedgene that has effect on ovulation rate.The test use Yunan black pig as target and American Duroc and Large White ascontrast,research on the2genes’ polymorphism and their relations with breedingperformance, to discuss application of the2genes as molecular target in Yunan black pigbreeding. Phenol-Chloroform extraction was used to get DNA of pig blood and Pig hairfollicle genome in the test.Construct DNA pools, Conduct NCOAI whole genomeSequencing, Screen Variable sites of Pig NCOAI gene, Adopt PCR-RFLP tech to analysepopulation genetics on NCAOI exon and SNP locus:G223A,T196C,T427C,G72A ofADAMTSI gene of346pigs from3species; Use SPSS17.0to analyse the influence of the4Genetic Variant on pig breeding properties. Main results are showed below:1. Analysis of.NCOAI genetic variation.Construct DNA pools with extracted piggenome DNA,to sequence mixed DNA pools for extreme properties.,found possiblepolymorphic locis exist in exon3,8and12,design primers respectively,use PCR-RFLPand detected3SNP locus in exon3,8and12is G223A,T196C,T427C.Sequencing resultindicate the3SNP locus is G/A,T/C,T/C mutation respectively. All3locis have2allelesand3genotypes. Genetic variation analysis shows, A(0.509) is advantage alleles onG223A.Advantage genotype is AB(0.624).The loci is on nonequilibrium condition ofHardy-Weinberg. Polymorphism Information Content (PIC), Heterozygosity (He),Effectivenumber of alleles(Ne) are0.375,0.500,2.000separately, belong to moderatepolymorphism; C(0.626) is advantage alleles on T196C.Advantage genotype is CD(0.460).The loci is on equilibrium condition of Hardy-Weinberg.PIC,He,Ne are0.359,0.468,1.881separately, belongs to moderate polymorphism;E(0.652) is advantage alleles onT427C.Advantage genotype is EF(0.517).The loci is on nonequilibrium condition of Hardy-Weinberg.PIC,He,Ne are0.351,0.454,1.831separately, belongs to moderatepolymorphism;Analyse with the Reproductive data that related with breeding propertiescorrelatively from the3SNP detected,results show:Extremely Significant Difference inTotal Number Born, number born alive, Was born litter weight among genotypes ofG223A(p<0.01);No Significant Difference in Total Number Born, number born alivebetween genotype CC and CD(p>0.05), Extremely Significant Difference with genotypeDD(p<0.01) respectively. Extremely Significant Difference in21age litter weight amonggentotypes(p<0.01); Extremely Significant Difference in Total Number Born, number bornalive among genotypes of T427Cs(p<0.01), same performance on Was born litter weightand21age litter weight: Significant Difference in EE and EF(p<0.05),both in ExtremelySignificant Difference with FF(<0.01).2. Analysis of ADAMTS1genetic variation.Design primers at the area of GeneticVariant of the7th exon of ADAMTS1to use for Amplification of the sequence thatcontain inserted segment, use PCR-RFLP to inspect Genetic Variant G72A.. Sequencingresult Shows, the SNP is G/A mutation and has2alleles and3genotypes. Geneticvariation analysis reveals, A(0.633) is advantage alleles on G72A.Advantage genotype isAB(0.457). The loci is on equilibrium condition of Hardy-Weinberg.PIC,He,Ne are0.357,0.465,1.868separately, belongs to moderate polymorphism;Analyse with theReproductive data that related with breeding properties correlatively from the GeneticVariant,results show:Both Extremely Significant Difference in Total Number Born,number born alive, between AA and AB,AA and BB of G72A(p<0.01),no SignificantDifference between AB and BB(p>0.05); Extremely Significant Difference in Was bornlitter weight and21age litter weight among genotypes(p<0.01).3. After LSD anylysis based on single factor with different locus and samegenotype,analysis of breeding related properties of Yunan black pig, Duroc, LargeWhite,we get: Large White has Extremely Significant Difference in Total Number Born,number born alive, Was born litter weight,21age litter weight with, Duroc and Yunanblack pig.(p<0.01);No Significant Difference in Total Number Born, number born alive,Was born litter weight between Duroc and Yunan black pig.(p>0.051) but ExtremelySignificant Difference in21age litter weight. Yunan black pig...