The Study On Seed Prinming And Preliminary Proteomic Analysis Of The Chloroplast Protein On Young Spikelet Initiation Stage With Thermo-sensitive Genic Male Sterile Maize9417

In this article,we have selected Thermo-sensitive Genic Male Sterile Maize asexperimental material. Application of seed priming technology and proteomicanalysis methods,respectively, the study Determination of the seed viability and somephysiological and biochemical indexes with different chitosan concentrationssolutions, the treatment effect of Seed Prinming was indicated with the relevantphysiological and biochemical indexes, To discriminant effective Seed primingapproach of Chitosan solution on Thermo-sensitive Genic Male Sterile Maize; theresearch had measured differential proteomic expression on sensitive period of spikedifferentiation process. Determined the physiological index of. sensitive period ofspike differentiation. To study corn fertility conversion mechanism of infertility withthe molecular proteomics level associated physiological variables. To as a Guidelinefor thermo-sensitive Genic Male Sterile Maize’s practical application.The main experimental results is following:The study of Seed Prinming: it was measured the vitality of the different chitosanConcentrations of the thermo-sensitivegenicmale sterile maize and somephysiological and bio-chemical indexes; The effect of Seed Prinming was revealed tby Physiological in dicators were analyzed and compared. It is to determine theconcentration of chitosan solution..After a comprehensive comparison, theconcentration of0.5%chitosan solution works best.The mechanism of maize fertility transition Establish the corn protein extractionmethod system:Filterout the corn gelelectrophoresis conditions, the proteinband with maximum to use extraction method of2, The14μL the sample size is thebest, Elect the appropriate gel concentration13%Coomassie brilliantblue staining issuitable for staining in this experiment, Young Spikeletproteindifferentially expressed proteins, i so lated in13%gel gel, Compare the differencesfree proline,soluble sugar and soluble protein content in Young Spikeleinitiation sensitive period leaves. Fertile lines and Sterile are different;Finally, four fertile protein were identified in Fertile lines and Sterile plantsof young Spikelet protein of the sensitive period, with mass spectrometry and bioinformatics analysis. The experiment was an I n-depth study o ftemperature-sensitive maize male sterility mechanism meaningful exploration.