Correlation Of Mutation In PPARGC1α Gene With Meat Traits In Sunit Sheep、Bamei Sheep、Small-tail Han Sheep

PPARGC1α is a nuclear transcription coactivator, abundantly expressed in brown adipose tissue, skeletal muscle and other parts of the distribution of white adipose tissue, pancreas.The analysis PPARGC1α gene expression of DNA sequences between Sonid sheep, the Pakistan America sheep, Han sheep in three different varieties to identify the gene mutation between the different varieties of PPARGC1α,with indicators of meat quality traits in association studies.1. Highly conserved region of the gene sequences of human, mouse, cattle, pigs and other species PPARGC1α find the nine groups to design primers for PCR reaction proved Sonid sheep, the Bamei sheep PPARGC1α genes of the segment.2. Analysis of the results of different primer sets Sunite sheep, the Bamei sheep, Han sheep PPARGC1α gene sequence PCR.Specimens from three sets of specific primers strong group removed six groups of non-specific or weak specificity of the primer set, PCR sequencing reaction.3. Sonid sheep, the Bamei sheep, Han sheep PPARGC1α gene sequences, found sonid sheep, the sheep of Bamei, between Small Tail Han sheep three sheep, a variety of point mutations. Compare Sunite sheep, the Bamei sheep, Small Tail Han sheep three sheep breeds sequence found7pr13primer sequence of669bp at the mutation sites7pr21primer sequence of87bp at the mutation sites,7pr40primers the amplified sequence of554bp at the mutation site.4.The Sonid sheep, the Bamei sheep, Small Tail Han sheep among three varieties7pr13primers amplified sequence of669bp at the mutation site,7pr21primers amplified sequence of87bp at the mutation site,7pr40primers amplified sequence of554bp at The mutation sites associated with the succulent shape indicators.