The Combination Effects Of Paracetamol And N-acetylcysteine On Antioxidant Capacity And Hematology Index Induced By Immunological Stress In Piglets

Weaned piglets are very easil y invaded b y p athogens and bacterialendotox ins due to their immune s ys tem and intestinal function areincomplete develop ment. Weaning stres s and immunological stress causedb y v accination and the foreign bod y rejection in feed are all likel y tocause oxidation stress. This stud y will explore the combinat ion effects o facetaminophen (AA P) and acet ylc yst eine (NAC) on antiox idant capacit yand hematolo g y index induced b y immunological s tress in pigletsEx periment one was conducted in vitro to stud y th e effects ofacetaminophen, acet ylc ysteine and th eir combination o n antiox idantcapacit y of pi glets peripheral blood m ononuclear cells an d chan ges ofrelevant ind ex es in the cell culture flu ids. The results sho wed that SODand GSH in cells of LPS-induced model group was ex tremel y different incontrast to the blank control group, but h ad litter chan ge in t he content ofMDA. SOD activit y and GSH concentrat ion in the LPS+AAP model groupgraduall y increas ed with APP concentration increase in contrast to theLPS model group, and the differen ce w as ex tremel y si gnificant when APPconcentration was added at0.5mM. T he index es of LPS+NAC modelgroup w ere ex tremel y different from th e LPS model group ex c ept NACconcentration added at0.0625mM. The effect of LPS+NAC+AAP modelgroup was better than the effect of the drug alone, and w as ex tremel ysignificant when N AC was add ed at0.5mM and APP w as added at0.625-0.5mM. Addition, the MDA content was relative l y stable, so there was no ex tremel y si gnificant differen ce in the cell culture fl uids.Ex periment two was conducted in vivo to investigate the impacts ofacetaminophen, acetyl c ysteine and their interaction on the i mmunologi calstress model established th rou gh intraperitoneal i njecti on withlipopol ys acch aride (LPS). The chan ges of antiox idant ca pacit y (SOD,GSH, GSH-PX,T-AOC, MDA) had detected. There were a total of30health y weaned pi gl ets wei ghing11-13kg. Firstl y th e y w ere fed no rmall yfor ten da ys. Pi glets were randoml y allo tted to five treatmen ts, including:①nonchallenged control;②challenged control (basal diet);③LPS+AAP(600mg/k g b asal di et);④LPS+NAC (1200mg/k g bas al d iet);⑤LPS+AAP+NAC. In the formal trial,①and②were fed b asal diet,③were fedAAP mix ed feed,④were fed NAC mix ed feed,⑤w ere fed AAP+N ACmix ed feed. After the prev entive admin istration of5da y s, piglets in the②to⑤groups were injected intraperitoneally with100μg/kg BW LPS,whereas piglets in①group w ere injected with an equivalent amount ofsterile saline. Compared with the control g roup, LPS challenge reduced(P<0.05) SOD ctivit y and GSH con centrations in plasma at3h post LPSinjection, while increasin g the con centrations of M DA (P<0.05).Paracet amol could increase pl asma SOD activit y of LPS-challen gedpiglets. N-acet yl c ys teine alo ne or in co mbination with paracetamol couldalleviate si gnificant l y (P<0.05) the abno rmal chan ges b y LP S-challen ged.However, there w as no signifi cant difference in various groups at24hpost LPS-challen ged.Ex periment three was conducted in vivo to ev al uate the anti-immunologi cal stress effects of acetaminophen, acet yl cystein e andtheir interaction, and an immunological stress model was establishedthrough intraperitoneal injecting with lipopol ysacch aride (LPS). Pigswere wei gh ed respectivel y on first da y and fifth da y m orning withoutfeedin g to calculat e the average daily gain. Feed cons umption wascalculated on fifth day to gain the total feed intake o f each group. Th ebod y temperature of pigs that had been i njected with LPS or sterile salinein three h ours was detected. And there was a reco rd on their bodiesfunction reaction condition. Blood samp les were collected at3h and24hto detect blood biochemistry index es (A LT, AST,A LP,TP,A LB,T BIL,GLU,BUN and CREA) and peripheral hemat olog y index es (WBC,NEU, LYM,MONO,EOS, BSAO, RBC,HGB, HCT and P LT). Th e results suggested thatLPS induced pi glet ph ysiolo g y reaction severel y, such as fever, vomiting,tremor and so on. combination of acetam inophen and acet ylcys teine couldimprove the produ ctive perfo rmance, in addition to alleviating theLPS-induced pigl et vomiting. The di fference of blood biochemistry andhematolog y index es of LPS-induced pi glets w as ex tremely si gnificant.The combination diets ha d certain s ynergistic effects on these index es.In a conclusion, co mbination of acetaminop hen and acet ylcysteineenhances ntioxidan t cap acit y of LPS-induced piglet s, improve bloodbiochemistry index es, and alleviate the LPS-induced pi glet vomiting.