SSR Markers Of Stripe Rust Resistance Genes In Wheat101-3

Wheat is one of the food cereal crop worldwide. In China, except rice, wheat is the most important crop. However, wheat stripe rust which is caused by Puccinia striiformis West.f.sp.tritici is epidemic, the serious yield losses was usually produced, and resulting in, huge economic losses, and threats the safe of wheat production. In addition, the quality of wheat was also affected by the fungal pathogen in great degree. With the changing of the farming way and climate, stripe rust occurred in China more widely. Although there are many measures to reduce the losses to some extend, application of resistant cultivars offers effective, economical safe and environmental friendship approach to eliminate the use of fungicides and reducing crop losses caused by stripe rust. Therefore, extensively searching, collecting and identifying new resistance resources and genes is the base of wheat improvement for disease resistance.Line101-3with high resistance to current epidemic stripe rust races was derived from hybrid progeny between common wheat variety, Mianyang11(MY11) and Haynaldia villosa. The stripe rust resistance of wheat101-3was controlled by a pair of single dominant gene, and located the gene on the chromosome1B by previous studies. In order to more precisely mark the resistance gene in line101-3, genetic analysis of stripe rust resistance were further studied by SSR and the hybrid generations between a stable wheat line08-798and line101-3were used as materials in present paper to obtained more tightly linked molecular markers with the resistance genes. Results were summarized as follows:1. The hybrid F1and F2generations were produced between101-3as resistant parent and08-798as susceptible parent. The F1plants were found to immune to stripe rust when the predominant mixed races of stripe rust were infected, and the segregation ratio for resistant and susceptible plants of F2population fitted good into the expected3:1. This result indicated line101-3carried an unknowing single dominant gene for stripe rust resistance. The resistance gene was temporarily named Yr101-3.2. To obtain molecular marker(s) tightly linked with stripe rust resistance in wheat line101-3, the marker population was founded by bulk segregation analysis (BSA) technique and microsatellite primers on chromosome1B were used to screen the resistant and susceptible bulks and the marker population. Two SSR markers, Xwmc156and Xgpw2162on chromosome arm1B, were found linked with Yr101-3. suggesting that the stripe rust resistance gene Yr101-3in wheat line101-3was located on the chromosome1B. which agreed with the results of previous study. Linkage analysis was conducted with the software Mapmaker3.0. Result showed that Yr101-3was linked with Xwmc156and Xgpw2162with genetic distance of4.3cM and5.9cM. respectively.3. Ten stripe rust genes with eight sites have been located on chromosome1B of wheat according to literatures. The comparison was carried out between Yr101-3and the ten resistance genes. The result inferred that line101-3might carried a new stripe rust resistance gene on the chromosome1B.