Study Of Pharmacodynamics And Mechanism Of Supplemantarv Sijunzi Decoction On Piglets

This experiment was conducted to study pharmacodynamics and mechanism of supplemantary Sijunzi Decoction (SJZD). The models of spleen deficiency were prepared with rheum officinale solution method. The general signs, immune function, anti-stress, gastrointestinal motility and absorption of spleen deficiency mice were measured and growth performance and blood biochemical index were detected in piglets.TestⅠ.100KUN MING mice (20±2g) were randomly divided into normal group, model group, supplemantary SJZD low dosage group, medium dosage group and high dosage group,20each group. The trial was conducted for18d. In the whole experiment period, all mice were induced with0.6ml/mouse in normal group. After making model successfully, physiologicalsaline with0.6ml/mouse, supplemantary SJZD with0.25g/20g,0.50g/20g and0.75g/20g were induced in model group, supplemantary SJZD low dosage group, medium dosage group and high dosage group respectively.The general signs of spleen deficiency mice were recorded throughout the whole trial. Body temperature and body weight of mice were measured and recorded before and after making models successfully, the4th day, and8th day after treatment. At the9th day, anti-stress ability, immune function, gastrointestinal motility and absorbtion function were detected.TestⅡ.48piglets (15±2days of age) were randomly divided into4groups (control group, GroupⅠ, GroupⅡ, GroupⅢ). At15days of age, piglets were drunk with tap water, tap water+0.5g/100mL supplemantary SJZD, tap water+1.0g/100mL supplemantary SJZD and tap water+1.5g/100mL supplemantary SJZD, respectively.The trial was conducted for30d.Body weight with an empty belly and surplus feed were weighted on15,29and43d, feed consumption, and average daily feed intake (ADFI), average daily gain (ADG) and the ratio of feed to gain (F/G) were detected.The results were as follows:Compared with model group, the signs of spleen deficiency mice were improved, body weight were significantly increased (p<0.01) and body temperature were increased (p>0.05) in supplemantary SJZD medium dosage group and high dosage group. Serum IgM levels, spleen index and thymus index were increased significantly (p<0.01or p<0.05) in supplemantary SJZD medium dosage group. Devouring percentage of macrophage cell was significantly increased in supplemantary SJZD medium dosage group and high dosage group (p<0.01), and devouring index of macrophage cell was significantly increased in various dosage of supplemantary SJZD (p<0.05), swimming duration and anti-hypoxia ability could be prolonged (p<0.01or p<0.05), gastric emptying percentage and relative percentage each small intestine propulsion were decreased in various dosage of supplemantary SJZD (p>0.05), D-xylose excretion contents and serum amylase activities were enhanced(p<0.05).Compared with control group, the ADG were increased, F/G and diarrhea rates were decreased (p<0.01) in various dosage of supplemantary SJZD of piglets. T-lymphocyte transformation rate were significantly increased in various dosage of supplemantary SJZD (p<0.01) on33,38d, and were significantly increased in supplemantary SJZD medium dosage group (p<0.01) on43d. The generation of hog cholera antibody could be promoted in various dosage of supplemantary SJZD, but there were no significant difference. Serum IgAlevels could be significantly increased in various dosage of supplemantary SJZD on38,43d, serum IgG levels were increased in supplemantary SJZD medium and high dosage group (p<0.01).In conclusion, Sijunzi Decoction can improve spleen deficiency symptom, enhance the levels of serum immune globulin, prolong the swimming duration, improve anti-hypoxia ability, increase devouring percentage and index of macrophage cell, increase immune organ index, thereby improve immune function and express the effect of anti-stress of mice. Sijunzi Decoction can adjust digestive system through reducing gastric emptying percentage and small intestine propulsion, increase serum amylase activities and D-xylose excretion contents.Sijunzi Decoction supplemented to the water can improve growth performance, decrease significantly diarrhea rates, enhance cellular immunity and humoral immunity function, prmote the growth and decrease the effects of weaning piglets. The best dosage of Sijunzi Decoction was1%.