| We performed fattening experiments, gene flow experiments, the normal and biochemical indexdetection, physiological parameters detection of blood before and after the injection of exogenous genes,PCR dection with extracted DNA from four different conditions for Transgenic pig with IGF1gene, atthe same time, we select the same species, similar time of birth, weight and health conditon as thecontrol. All these experiments were perexcuted in the same breeding management, immunizationprograms, feed formulation, and other external conditions. And all the field test and lab test were carriedin the original pig breeding farm and lab of Hubei Academy of Agricultural Sciences, Institute ofAnimal Husbandry and Veterinary, expectively.The test results show that fattening turn IGF-I gene pig average daily gain (ADG) higher than22g,lean meat percentage of the experimental group than the control group improved9%, the data afterstatistical analysis of significant difference compared with the control group.From weaning to90kg slaughter pigs and control pigs difference in total cholesterol, triglyceride, totalprotein, albumin, total bilirubin, direct bilirubin, globulin, white globulin ratio and blood sugarmeasuring indicators are not significant.After7days, blood tests before and after the injection of exogenous genes, the percentage oflymphocytes, the percentage of intermediate cells, lymphocytes, neutrophil percentage, hemoglobin,erythrocyte count, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, redblood cell distribution width coefficient of variation, platelet hematocrit indexes by external stimuli, theperformance trend remained the same.Fecal sample injection7days before and after the acquisition of exogenous gene, placed in thenatural environment, each sample interval24h,48h,72h,96h, respectively, sample1, all samplecontinuous acquisition of a month. PCR results were not detected in the target gene drift.Control pigs and test-positive pigs in the same column or stall feeding to90kg, collecting bloodand stool were detected by PCR, were not detected in the target gene drift. |
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