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Anatomy And Mobility Studies For The Low Pesticide Residue In Cucumber Varieties

Posted on:2014-02-03Degree:MasterType:Thesis
Country:ChinaCandidate:W ZhaoFull Text:PDF
GTID:2233330398453733Subject:Vegetable science
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As non-staple food, vegetables play important roles in the human life. With the raise of livingstandard and understanding, vegetable’quality is more strict.So pesticide residue problem become moreand more prominent and necessary in all the vegetables. The vegetable crops, especilly the cucumber,are vulnerable to the threat of pests and diseases. In order to guarantee the production, the plants need tospay the large amounts of the pesticide. With the rapidly increase of pesticide requirement, lead to thebring out in the problem of pesticide residues. Pesticie residues affect export and people’s health. Inorder to ensure the quality of vegetable production, implement green barriers, it is important for us tostudy pesticide residues.A low pesticide residues cucumber (C. sativus L.) cultivar D0351and a high pesticide residuescucumber cultivar D9320which screened out of the number of germplasm resources in this group.Botany morphology features was made premliminary identification. Eastablished the evalution indexsystem and genetic analysis of cucumber germplasm resources about the low pesticides. Resultsconfirmed that the low pesticide varieties have genetic properties. By using electron microscopytechnology to the anylisis of charcteristic, anatomical structures the leaves, stems and epicarp areobserveed. By the method of control pesticide processing, we study transport path and transimission rateof pesticide liquid in the plants.Main results were as follows:Determine low pesticide residues varieties organization anatomical features1. The low pesticide residue varieties D0351are blade surface folds less, interval with large andsurface smooth. Stomatal fits on the leaf, is slightly concave, porosity small. Stem epidermal cells oflow pesticide residues varieties closely spaced, circular. Pericard cells of low pesticide residues varietiesis elongatde. The high pesticide residue varieties D9320are blade surface folds. Fold spacing is small.The surface is uneven exhibition. Stomatal processus on the leaf and porosity small. Stem epidermalcells of high pesticide residues varieties closely spaced, oval. Pericard cells of high pesticide residuesvarieties is square.2. Determine the tissue anatomy changes under pesticide treatmentEpidermal cells folds become less. The length of the pores are increased.stomatal opend are largerthan formal cultivar. The cells of upperepidermis and palisade tissue become more thicker. The degreeof enlargement of stem epidermal cells erratic change. Pericard cells changed in shape and size. The way of existence of stomatals have changed. The chloroplast internal osmiophilic particle numbers areincreased.3. Determine pesticides conduction wayUnder the two kinds of pesticide processing have the same transport way in two residue ofdifferent varieties. Chemical pesticide absorption from the leaves first transfer petiole, from petiole intothe stem conducting tissue, then transfer carpopodium and accumulated in the pedicel, then enter thehusk of the fruit, followed by entering the flesh, and finally reach the fruit flesh. Roots is absorbed fromthe soil chemical agents, the other part from the plants were conducted into the root.4. Determines the heigh of each part of the plant residues and the conduction rate orderChlorpyrifos processing, applying pesticide residue for high and low order two varieties: Leaf>Carpopodium> Stem> Petiole> Root>Peel> Pulp> Fruit flesh. Sequence of spaying pesticide residueprocessing two varieties: Leaf>Peel> Carpopodium> Root>Stem>Petiole> Pulp> Fruit flesh.Under the propamocard processing, the leaves are daubed the sequence of pesticide residueprocessing two varieties: Leaf> Carpopodium> Stem> Petiole> Root>Peel> Pulp> Fruit flesh. Spayingprocessing: Leaf> Carpopodium> Peel> Petiole> Stem> Pulp> Fruit flesh.The tendency of the transimission rate is the same in two stains under the two different pesticides.Pesticide conductivity constantly reduce by stem and petiole. Peel, pulp and fruit flesh reach mixmizingat24h.Chemical pesticides transduction is a two-way in vivo of plants.
Keywords/Search Tags:Cucumber, Low pesticide residue, Ultrastructure, Pesticide mobility
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