The Molecular Identification Of Ganoderma Lucidum Etc.9 Edible And Medicinal Fungi

In this study, we weed out strains which had the different names within the same species or in different specieses, and we also found a molecular approach for identifying species. ITS consequence and ITS-RFLP technology were used in the weeding. The 367 tested strains which was collected by Fujian Mycological Research Center, were classied into 10 specieses.1.Based on the ITS consequence, we set a ITS identifying approach and did a careful survey to the dismatch of strains to their names.2 . By combining the restriction fragment length polymorphism(ITS-RFLP) obtained from digestions with 7 enzymes AluI、HindIII、HaeIII、MaeI、XhoI、MspI and AccII, the 367 strains belonging to 10 specieses were classified. The results from ITS-RFLP were corresponded with morphological characters. The results are as follow:There is an unknown strains blended in these 7 Coriolus versicolor, 2 in the 66 Coprinus comatus, 1 in the 37 Hypsizigus marmoreus, 1 in the 14 Dictyophora, 1 in the 123 Ganoderma lucidum, 1 in the 9 Clitocybe maxima. And the 7 Coriolus versicolor can be sorted into 3 categories by the result of HindIII digested, 2 categories of the 66 Agrocybe chaxinggu by the result of AccII digested, 4 categories of the 19 Oudemansiella radiate by the result of MspI digested,.4 categories of the 123 Ganoderma lucidum by the result of MspI�'�HaeIII digested. And we also identify the 6 Wolfiporia cocos as Poria cocos, 1ergot as Fusarfum tricinctum by the analysis of ITS sequences.We take ITS sequence and ITS-RFLP technology into analyzing the relationship between species and within .And we also can tell by the result that ITS-RFLP has good stability and repeatability, and will be guidable significance for fast identification of edible mushroom specieses.