Study On Technique Of Molecular Identification Of Edible Fungi Pleurotus Spp.

The genus Pleurotus is a kind of wide spread wood saprobiotic fungus with edible and medical function,and its production is growing fast in recent years,while its taxonomy is confused.So,it is great significance to found a identified technology for Pleurotus species.In this study,we utilize the ITS sequences and ITS-RFLP analysis for studying the molecular identified technology among the 559 Pleurotus strains,which belong to 12 species collected by Fujian Edible Fungi Germplasm Resource Collection Center,and weed out the strains which had the different names in the same specie or the same name in the different species,also we found a molecular identified approach for Pleurotus species.The results are as follows.1.The length of ITS(including 5.8S rDNA) region ranged from 630-667bp.The length in pleurotus pulmonarius and Pleurotus djamor were 630bp and 667bp,respectively.Based on the ITS sequences analysis,12 Pleurotus species could be assigned to 8 kinds,including 5 single species(P.cystidiosus,P.citrinopileatus,P.djamor,P.tuber-regium,amd P.eryngii) and 3 complex groups(P.pulmonarius,P.nebrodensis and P.ostreatus complexes).The 8 kinds correspond with biological species reported.P.cystidiosus is meaningless in practical production.2.By combining the restriction fragment length polymorphism(ITS-RFLP) obtained from digestions with 7 enzymes AluⅠ,DraⅠ,EcoRⅠ,HaeⅢ,MaeⅠ,Sau3AⅠand SphⅠ,the 585 strains belonging to 12 Pleurotus species were efficient classified.The results from ITS-RFLP were corresponded with morphological characters.Based on the ITS size,AluⅠ-RFLP,HaeⅢ-RFLP and MaeⅠ-RFLP,we found a molecular identified technology for Pleurotus species.3.We investigated 7 probable Pleurotus strains in ITS size,AluⅠ-RFLP,HaeⅢ-RFLP, MaeⅠ-RFLP and DraⅠ-RFLP based on the molecular identified technology for Pleurotus species.7 strains were efficient identified.It is conferred that ITS-RFLP was veracity for identifying Pleurotus species by comparing the ITS sequencing.The result shows ITS-RFLP can be applied in identifying Pleurotus species.4.With the result of ITS sequence and ITS-RFLP patterns,the lineage relationship among 12 Pleurotus species are analyzed.The result showed that P.cystidiosus,P.citrinopileatus,P. djamor,P.tuber-regium was more distant from other Pleurotus species.P.abalonus and P. cyslidiosus are the same species,as well as P.citrinopileatus and P.cornucopiae,P.djamor and P. salmoneostramineus,P.pulmonarius,P.sajor-caju and P.geesteranus,P.floridanus and P. ostreatus.Furthermore,ITS-RFLP has good stability and repeatability,and will be guidable significance for fast identification of Pleurotus species.