Significance Of Renal Interstitial Mast Cells Infiltration In The Hepatitis B Virus Associated Nephritis

[Objective]:1. To compare the difference between the infiltration degree of renal mast cellsmarked by C3aR and tryptase by two different methods in the hepatitis B virusassociated nephritis, in order to raise the accuracy of renal mast cells detection.2. To observe the infiltration of tryptase-positive mast cells and the expression ofMCP-1in renal interstitial of the hepatitis B virus associated IgA nephropathy(HBV-IgAN) and primary IgA nephropathy(pIgAN), and investigate the correlationamong mast cells infiltration, degree of tubulointerstitial damage and expression ofMCP-1, and explore the role of MC infiltration in the progress of HBV-IgAN andpIgAN.[Methods]:1. The distribution of C3aR and tryptase positive-mast cells in renal biopsyspecimens of HBV-IgAN(n=6) and pIgAN(n=6) patients were determined byimmunohistochemistry, compare the differences between the two renal interstitialmast cells detections.2. Renal biopsy specimens from thirty HBV-IgAN and pIgAN patients to beobject of the study, normal renal tissue sections from6nephrectomized patients fornephroma to be normal control．The infiltration of tryptase positive-mast cells andThe expression of MCP-1in renal biopsy specimens were determined byimmunohistochemistry, tested by semi-quantitative method. The relationship amongthe expression of mast cells and MCP-1and the clinicopathologic data was analyzed.[Results]:1. C3aR and tryptase-positive mast cells mainly distribute in renal interstitial,highly consistent in morphology, distribution area are similar, but do not completelyoverlap. No significant difference between the two methods detecting number of mast cells(P>0.05) which are highly correlated. The infiltration number of C3aR andtryptase-positive mast cells increased as compared with the control group, and nodifference between HBV-IgAN and pIgAN.2. Numbers of renal interstitial mast cells increased with the elevation ofpathological grading in HBV-IgAN and pIgAN(P<0.05). Compared with controlgroup, The number of MC began to increase in the level II of pIgAN group, but thelevel II of HBV-IgAN group was not significantly affected. The number of MCincreased with the aggravation of tubulointerstitial damage, no significant differencebetween no-tubulointerstitial damage group of HBV-IgAN and pIgAN compared withcontrol group, or between the two groups of diseases(P>0.05). In addition, Thenumber of renal interstitial MC was positively correlated with serum creatinine（r=0.498,0.505,P<0.05) in the two diseases.3. The expression of renal interstitial MCP-1increased with the aggravation oftubulointerstitial damage and the elevation of pathological grading in HBV-IgAN andpIgAN. Compared with control group, The expression of MCP-1in the level II groupand no-tubulointerstitial-damage group of both diseases were not significantlychanged(P>0.05). And no significantly difference between the two diseases(P>0.05).4. There is a positive correlation between the infiltration number of mast cellsand the expression of MCP-1and among them with tubular atrophy, interstitialfibrosis and interstitial inflammatory cells. No significantly difference between thetwo diseases(P>0.05).5. Compared with NS group of pIgAN, mast cells increased and MCP-1up-regulated and the degree of tubulointerstitial damage aggravated in NS group ofHBV-IgAN(P>0.05).6. The aggravation of tubulointerstitial damage and the elevation of pathologicalgrading were observed in HBV-IgAN and pIgAN with the increasing numbers of MC.[Conclusion]:1. The two methods of C3aR and tryptase in detecting renal mast cells arefeasible, could both be reliable detections of renal interstitial mast cells infiltration. 2. There is a positive correlation among renal interstitial tryptase positive-mastcells infiltration and tubulointerstitial damage and serum creatinine in HBV-IgAN andpIgAN, the degree of MC infiltration was no significantly difference between the twogroups of diseases. Suggested that MC infiltration was probably one of the commonpathway of tubulointerstitial damage of HBV-IgAN and pIgAN.3. There is a positive corelation between renal interstitial mast cells infiltrationand MCP-1expression which were positively involved in tubulointerstitial damage ofHBV-IgAN and pIgAN. Suggested that MC mediated tubulointerstitial damageprobably by autocrining MCP-1.