Studies Of Serum Levels Of Uric Acid In Infections Of Central Nervous System And In Vitro Study Of Correlations And Expression Of IL-10and TNF-α After Human PBMCs Contaminated With BCG

ObjectivesThe aim of this study is to explore the relationship between serum uric acid(SUA) and development or activities of central nervous system infections (CNSI),which may be helpful to provide new ways for condition assessment and clinicalintervention of CNSI.MethodsThe SUA levels were measured in357patients with different types of CNSI(bacterial meningitis or meningoencephalitis, viral meningitis or meningoencephalitis, tuberculous meningitis or meningoencephalitis,cryptococcus meningitisor meningoencephalitis, and cysticercosis of brain, respectively) and132patientswith multiple sclerosis (MS), and89healthy controls (HC). Each group of CNSI wasadministered with relevantly conventional therapy(main treatments were as fallows:bacterial meningitis or meningoencephalitis: Rocephin etal; viral meningitis ormeningoencephalitis:Valaciclovir Hydrochloride tablets etal; tuberculous meningitis or meningoencephalitis: Isoni cotinyl hydrazide、Rifampicin、Ethambutol、Aldinamide(PZA)and Moxifloxacin Hydrochloride injection etal; cryptococcusmeningitis or meningoencephalitis: Amphotericin B、5-FU、Diflucan etal; cysticercosis of brain: Albendazole etal).SUA levels were compared before and afterrelevantly conventional therapy using a automatic biochemistry analyzer.ResultsSUA levels of5groups patients with CNSI（221.34±120.08μmol/L）weresignificantly lower when compared with healthy subjects(312.06±92.76μmol/L,p＜0.001)，equally lower than MS（244.48±111.86μmol/L,p=0.051）.SUA levels ofpatients with MS were significantly higher when compared to the patients with bacterial meningitis（170.22±102.02μmol/L, p=0.000）、tuberculous meningitis ormeningoencep halitis（200.04±129.81μmol/L, p=0.006）、cryptococcus meningitisor meningoencephalitis (179.59±106.77μmol/L, p=0.000); while there was nosignificant difference when compared with viral meningitis or meningoencephalitis(264.94±120.67μmol/L, p=0.173) and cysticercosis of brain (264.86±96.97μmol/L,p=0.217). That is to say, SUA levels of MS subjects were very close with patientssuffering from viral meningitis or meningoencephalitis and cysticercosis of brain, butwere obviously higher than bacterial meningitis、tuberculous meningitis or meningoencephalitis and cryptococcus meningitis or meningoencephalitis, which indicatedthat the SUA concentration of CNSI was at lower level; besides, SUA levels of eachgroup of CNSI were significantly higher when compared to the patients with thosebefore treatment.ConclusionsOur data showed that SUA levels of5groups of CNSI were low, and SUA levelswere significantly higher after treatment than before. ObiectiveTo investigate the relationship and expression of IL-10(cytokine Interleukin-10)and TNF-α (Tumor necrosis factor alpha) after PBMCs (peripheral bloodmononuclearcells) contaminated with BCG (Bacille Calmette–Guerin).MethodsWhen PBMCs were infected with BCG, after3h、6h、8h、24h respectively，mRNA levels of IL-10and TNF-α were assayed using Real-time Quantitative PCR(RT-PCR); besides, four time points (8h、20h、32h、48h) were set after PBMCscultured with anti-IL-10antibody. Tne protein expression levels of IL-10and TNF-αin surpernatant were determined by enzyme-linked immunosorbent assay (ELISA).ResultsIL-10and TNF-α levels released by PBMCs were significantly increased afterstimulation by BCG in mRNA molecular and protein expression levels（p﹤0.05）, asignificantly negative regression was found between them; TNF-α levels producedwere significantly higher than the control group after adding exogenousanti-human-IL-10（P﹤0.05）.ConclusionsBCG could induce the expression of IL-10and TNF-α in PBMCs increasedsignificantly，and IL-10could inhibit the productions of TNF-α. Therefore, our paperprovide a basis for study new type anti-tuberculosis vaccines and tuberculosisimmunological pathogenesis.