Expression And Significance Of Tight Junction Protein Claudin-7in Renal Cell Carcinoma

Objective Be means of test the expression of tight junction proteins claudin-7mRNA and claudin-7protein in renal cell carcinoma (renal cell carcinoma, RCC) andexplore its relationship with clinic pathological features,probe the occurrencemechanism of RCC.Method Using in situ hybridization (in situ hybridization histochemistry;ISHH) and immune histochemistry (immunohistochemistry), respectively, from themRNA and protein levels was detected in49cases of renal cell carcinoma (studygroup) and45cases of normal renal tissue (control group) in the claudin-7geneexpression and analysis of its relationship with clinicopathological parameters in renalcell carcinoma.Results The observation group and control group of claudin-7mRNAexpression rate were53.06%,100%, P <0.05; The observation group and controlgroup of claudin-7protein expression rates were44.90%,100%(P <0.05). claudin-7mRNA and claudin-7protein expression in renal cell carcinoma T stage and clinicalstage related with gender, age, pathological type, tumor size, Fuhrman grade, lymphnode metastasis and distant metastasis. claudin-7mRNA and claudin-7proteinexpression in renal cell carcinoma T stage and clinical stage related difference wasstatistically significant (P <0.05). Nothing to do with gender, age, pathological type,tumor size, Fuhrman grade, lymph node metastasis and distant metastasis, thedifference was not statistically significant (P>0.05). Conclusion1、The expression of claudin-7mRNA and claudin-7protein inRCC were all lower than normal kidney tissue，prompting that claudin-7may beinvolved in the development and progression of renal cell carcinoma，and abnormalregulation before the translation level may be its mechanism.2、With the T stage andclinical stage increased，The expression of claudin-7mRNA and claudin-7protein inRCC reduced，prompting claudin-7play a tumor suppressor function in RCC,and itsabnormal expression can be a RCC development Prognostic factors.