Biocompatibility Of The Biomaterial

Nano science and technology was born in the late 1980s. For decades, it develops extremely rapidly and covers almost all fields of science. Especially in life science, it has become one of the most attractive and vitality directions. As applied to bioscience, especially used in drug treatment, nanobiomaterials performance well in many aspects such as biological compatibility, biodegradation, drug slow-release, drug bioavailability and targeted transfer. In this paper, we’ll do some works to test and judge a series of nanobiomaterials in these aspects preliminarily. The main research results are as follows:1. Through the four thiazole salt （MTT） colorimetric method which used cervix cancer cells Hela and human liver cancer cells Bel7404 for test cells, changing sample concentration, duration and cell types etc. respectively, we examinated and judged the biological compatibility of the water-soluble core-shell quantum dot CdSe/CdS-NH₂ or CdSe/CdS-COOH modificated from quantum dot CdSe/CdS. The results found that the negative effects of the cell’s growth by either quantum dot CdSe/CdS-NH₂ or CdSe/CdS-COOH was increased with concentration’s increasing, was decreased with the extension of putting time of the samples. And the negative effects by the quantum dot CdSe/CdS-NH₂ was much more than the quantum dots CdSe/CdS-COOH. In addition, Hela cells were more sensitive to these two samples than Bel7404 cells in this test.2. Through conventional MTT method, we investigated the biocompatibility of the ABC triblock copolymer of poly（ethylene glycol） monomethyl ether-b-poly（ε-caprolactone）-b-poly（L-lactic acid） and ABCBA pentablock copolymers of poly（L-lactic acid）-b-poly（ε-caprolactone）-b-poly（ethylene glycol） monomethyl ether-b-poly（ε-caprolactone）-b-poly（L-lactic acid）, or the cell’s vitro activity inhibition of the complexes formed with those triblock copolymers or pentablock copolymers and good drugs doxorubicin half lactose targeting liver cells,using cervix cancer cells Hela and human liver cancer cells Bel7402. We primarily validated their good drug slow-release and targeted release effect. The results show that both these blank nanoparticles had a very good biocompatibility. The cell’s death rate of doxorubicin drug-loading nanoparticles was far below the pure doxorubicin and the cell death rate of complexes linked with targeted groups of half lactose drug-loading nanoparticles was significantly greater than the untargeted drug-loading nanoparticles, which indicated that nanoparticles had a good drug slow-release and targeted release effect.3. Through conventional MTT method using human liver cancer cells Bel7402, we investigated the biocompatibility of several kinds of copolymers such as the triblock copolymer poly（L-lactic acid）-b-poly（L-lysine）-b-poly（ethylene glycol）, poly（L-lactic acid）-b-poly（Nε-carbonyl benzyl oxygen-L-lysine）-b-poly（ethylene glycol）, poly（ethylene glycol）-b-poly（L-lysine）-b-poly（L-lactic acid）, and poly（ethylene glycol）-b-poly（Nε-carbonyl benzyl oxygen-L-lysine）-b-poly（L-lactic acid）, or the diblock copolymers methoxy-poly（ethylene glycol）-b-poly（ε-caprolactone）, tetraethoxy-silicone -poly（ε-caprolactone）, and the tetraethoxy-silicone-poly （Nε-carbonyl benzyl oxygen-L-lysine）. The results show that the growth suppression of the Bel7402 cells done by these copolymers was proportional to the concentrations and the incubation time. And if the added concentration of these polymers is within 10μg/mL, the cell’s death rate will be very low. In short, they all had an excellent biocompatibility and reached the requirements of biological safety primary, so that we can use them in biological medicine and make a further study.