| In order to provide theoretical foundation for developing new food resource, the present paper used rhizome of Rhodiola rosea as raw material to study separation and purification techniques of proanthocyanidins, and also conducted assays investigating its anti-oxidant functions and anti-tumor effects.The main researches are as follows:(1) Study of purification of Rhodiola rosea proanthocyanidins. Firstly, Rhodiola rosea was extracted by ethanol, before the extract was purified by acetic ether leaching and AB-8 macro porous resin absorption. Based on former researches about sample concentration, flow velocity of sample loading and concentration of desorption solution, the present study optimized purification technique of AB-8 macro porous resin absorption as the sample liquid pH、desorption solution pH and diameter to height ratio. The optimized conditions of leaching, absorption and desorption were explored, and samples of oligomer and polymer with high purity were achieved.(2) Researches on anti-oxidative effects of Rhodiola rosea proanthocyanidins. The eliminating effects on·OH,·DPPH,·O2 produced by Pyrogallol auto-oxidation and NO2- of Rhodiola rosea proanthocyanidins were evaluated by tests in vitro. And integral animal experiments were employed to observe Rhodiola rosea Proanthocyanidin oligomers’influence on SOD, GSH-Px,and MDA levels of peripheral blood, liver, heart and brain.(3) Study on anti-tumor property of Rhodiola rosea proanthocyanidins. The S180 tumor–bearing animal model was established, and we monitored the development state of sarcoma in animal body; According to the average tumor heavy,the inhibitory rate (refers to Rhodiola rosea proanthocyanidins’function on inhibiting tumor growth) was calculated.The main results are along these lines:(1) The optimized conditions of acetic ether leaching were: extracting time of 25 min, volume rate (acetic ether to extractant) of 1.5:1, operation times of 4. The optimized conditions of AB-8 macro porous resin absorption were: sample liquid pH of 4.5, desorption solution pH of 5, and diameter to height ratio of 1:40 (cm). Under the above optimized conditions, Rhodiola rosea Proanthocyanidin was separated and refined, with product purity reaching 88. 3%, and polymer product purity was 98%.(2) Rhodiola rosea proanthocyanidin had strong ability of diminishing·OH, DPPH·,·O2 and NO2-, and the outcome was better than ascorbic acid. After administration of Rhodiola rosea proanthocyanidins, activities of SOD and GSH-Px in mouse blood, heart, liver and brain improved, while MDA content declined; 200 and 400 mg/kgbw dose groups notably increased GSH-Px activity and decreased MDA content in the mentioned 4 tissue samples, as significant differences were observed in comparison to the control group(P<0.05); however, SOD activity in liver showed no significant difference between dose groups and control group(P>0.05), while in the other 3 tissue samples, SOD activities in dose groups were significantly different from the control group(P<0.05).(3)Rhodiola rosea proanthocyanidin oligomer unfolded some degree of anti-tumor activity, which was dissimilar among mice of different genders. The average tumor weights of male mice from low, middle and high dose groups obviously reduced, and displayed significant differences in contrast to the control group, the inhibitory rates (refer to Rhodiola rosea proanthocyanidins’function on inhibiting tumor growth) were 47.5%, 51.4% and 56.9% separately. However, though average tumor weights of female mice from dose groups were less than the control group, no significant difference was revealed in the data, and inhibitory rates were 24.4%, 40% and 41.1% separately.The results indicated that the separation method of acetic ether leaching along with AB-8 macro porous resin absorption had admirable effects, and Rhodiola rosea proanthocyanidin products were with high purity. Through the anti-oxidative experiments and anti-tumor explorations, Rhodiola rosea proanthocyanidin oligomer was proved to be an excellent natural antioxidant with some degree of anti-tumor activity. |
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