| Objective :To observe the protective effect of rhodiola rosea on vascular endothelium in intermittent hypoxia(IH)rats and explore its possible mechanism.Methods :45 male SD rats were randomly divided into normal control group,intermittent hypoxia group and rhodiola low,medium and high dose group.The IH model was reproduced by putting the rats into the IH model chamber,and then feeding them with nitrogen,oxygen and compressed air for 45 days.The feeding bin and feeding time of rats in the normal control group were consistent with those in other groups,and the oxygen concentration in the tank was maintained at 20%~21%.Rhodiola rosea high,medium and low dose groups were intraperitoneally injected with rhodiola rosea 0.2ml,0.1ml and 0.05 ml /100 g starting from the 15 th day of IH,and continued for 30 days.The levels of malondialdehyde(MDA),superoxide dismutase(SOD)and nitric oxide(NO)in the coronary arteries of rats in each group were measured by automatic biochemical analyzer.The content of coronary hypoxia-inducible factor-1?(HIF-1 ?)and tumor necrosis factor-?(TNF-?)in rats were determined by ELISA.The expression levels of endothelin-1(ET-1)and vascular endothelial growth factor(VEGF)in coronary artery tissues of rats in each group were measured by reverse transcription-polymerase chain reaction(rt-pcr).Pathological changes of coronary arteries were observed under light microscope.Results :Compared with the normal control group,SOD and NO in the IH group decreased(SOD(U/mg): 4.43±0.22 vs.8.60±0.34,NO(umol/g): 3.09±0.07 vs.4.81±0.41),MDA,TNF-?,HIF-1?,ET-1 /GAPDH,VEGF/GAPDH increased(MDA(nmol/mg): 0.78±0.03 vs.0.50±0.03,TNF-?(pg/mg):6.35±0.29 vs.3.27±0.14,HIF-1?(ng/mg): 14.55±0.70 vs.7.16±0.17,ET-1 /GAPDH: 1.75±0.03 vs.1.10±0.07,VEGF/GAPDH: 4.38±0.10 vs.1.20±0.07,all P < 0.01.Compared with the IH group,SOD and NO were increased in each dose group,and MDA,TNF-?,HIF-1?,ET-1/GAPDH and VEGF/GAPDH were decreased in each dose group.The changes in the high-dose rhodiola group were more significant than those in the low and medium dose rhodiola group(SOD(U/mg): 7.47±0.19 vs.5.41±0.37,6.71±0.28,MDA(nmol/mg): 0.57±0.20 vs.0.74±0.04,0.70±0.03,NO(umol/g):4.00±0.28 vs.3.27±0.18,3.47±0.28,TNF-?(pg/mg): 3.90±0.17 vs.5.08±0.27,4.39±0.26,HIF-1?(ng/mg): 8.40±0.23 vs.11.07±0.41,9.81±0.44,ET-1 /GAPDH: 1.12±0.04 vs.1.71±0.03,1.63±0.07,VEGF/GAPDH:2.45±0.09 to 3.99±0.12,3.27±0.08).Under light microscope,the innermembrane of the normal control group was intact,and the endothelial cells were loose and slightly stained on the surface of the inner membrane.In the IH group,part of the arterial areas showed endointima edema or even abscess,interstitial edema of the vascular wall.The pathological changes of rhodiola roseain each dose group were less than that in the IH group,and the changes of rhodiola rosea in the high-dose group were more significant.Conclusion::Rhodiola rosea can protect the vascular endothelium caused by IH exposure by improving the level of anti-hypoxia in tissues,inhibiting oxidative stress and inflammatory response. |
PDF Full Text Request