| Objective To investigate the mechanism of apoptosis following renal ischemia/reperfusion(I/R) in rats and the protective effect of Pyrrolidine dithiocarbmate (PDTC).Methods Healthy male Wistar rats were randomly divided into three groups:①I/R group: established rats renal ischemia-reperfusion model;②PDTC group:PDTC-primed with100mg/kg;③sham-operated group. Respectively, rats were sacrificed at different time points after reperfusion (0h,2h,8h,24h). Serum creatinine (Cr) and urea nitrogen (BUN) were determined, renal pathological alterations were observed by HE staining. The expression of NF-κB and caspase-3were detected by immunohistochemistry and western blot technique, the iNOS activity and NO content of kidney tissues was detected by ELIS A. The apoptotic index was detected by TUNEL technique.Results In I/R group, the serum levels of Cr and BUN were increased significantly, HE staining indicated that tubular dilatation and interstitial inflammation, changes in PDTC group were alleviated significantly. In I/R group, NF-κB expression was significantly increased at2h after reperfusion, reach the peak at8h, mainly expressed in the nucleus, began to decline at24h. The expression of iNOS, NO, caspase-3were increased at2h, keeping escalating trend at observation points, simultaneously apoptotic index was significantly increased, they all have statistical significance as compared with sham-operated group and PDTC group (p<0.05). In PDTC group, the expression of all above parameters was increased at2h, reached the highest at8h, they all have statistical significances as compared with sham-operated groups(p<0.05), no significant differences were found at24h.Conclusions Renal ischemia/reperfusion can induce renal injury and apoptosis, PDTC may play a significant protective effect by decreasing NF-κB-induced NO formation. |
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