The Study On Relationship Between Gene Polymorphism Of Cyclooxygenase-2as Well As Environmental Risk Factors And Familial Clustering Of Primary Liver Carcinoma In The Guangxi

Objective To study the relationship between the polymorphism of cyclooxygenase-2(COX-2)as well as environmental risk factors of the cancer and familial clustering of primary liver carcinoma (PLC) in Guangxi.Methods Specimens contains124members of12PLC clustering families and129members of12control families of Guangxi population,with very high incidenc rate of PLC.According to the epidemiology of designed questionnaire, the method of field investigation was to collect relevant material, simultaneously collecting10ml eripheral blood the research object each. Detection of HBV infection markers, the gene chip technology detecting the genotype of COX-2rs689466and rs20417, comparative analysis the correlation between the two families for different genotype,environmental factors and familial clustering of primary liver carcinoma.Results (1) high-risk famlies of positive HBsAg rate accounted for37.1%(46/124), control families of positive HBsAg rate11.6%(15/129).Two familiess of HBsAg positive rate distribution difference were statistically significant (X2=22A14,P=0.001), the relative risk OR=4.482(95%Cl=2.340～8.586),the results of HBV infection rates in high-risk famlies as follow, proband,first-degree relatives,second-degree relatives,third-degree relatives were100%(12/12),37.6%(18/46),28.9%(13/45)及23.8%(5/21), indicating that the HBV infection rates level sequence was relatives first-degree relatives>secong-degree relatives>third-degree relatives.The results of chi-square trend inspection wasX2=14.215,P<0.001.(2) Rs689466genotype GG, GA, AA distribution in high-risk families14.52%(18/124),53.23%(66/124),32.25%(40/124),in the control families were39.54%(51/129),54.26%(70/129),6.20%(8/129), AA in two families of distribution difference had statistical significance(X2=37.149,P=0.000). rs20417in the control families and the high-risk families distribution agreement, there was no statistical significance.(3) Frequencies of rs689466allele A in high-risk families was58.87%(146/248), but in control families was33.86%(86/258), frequencies of allele A in high-risk families frequency was greater more than the control families; allele G in high-risk families was41.13%(84/248), but in control families was66.04%(172/258),A,G in two families of distribution difference had statistical significance (X2=26.973,P=0.000).(4) Frequencies of rs689466allele A in proband, first-degree relatives, second-degree relatives, third-degree relatives were54.17%(13/24),69.56%(64/92),55.56%(50/90),45.23%(19/42); Frequencies of allele G in proband, first-degree relatives, second-degree relatives, third-degree relatives were45.83%(11/24),43.18%(28/92),44.44%(40/90),45.23%(23/43); Allele A relative level with the decrease of relatives-degree was lower, but allele G was oppsition.correlation existed between relatives level and distribution of allele A and G (X2=8.197,P=0.042).(5) After analysis by the non-condition logistic regression,we found that COX-2rs689466genotype,positive HBV infection in serum and corn intake is the major high risk factors, it found that the COX-2rs689466gene polymorphism, HBV infections and corn intake were probably the major risk factors of familial clustering of primary liver carcinoma.Conclusion The polymorphism COX-2, HBV infections and corn intake is may associate with the familial susceptibility to primary liver carcinoma.