| Objective:To investigate the genetic susceptibility of HLA-DQA1,-DQB1alleles to polymyositis/dermatomyositis in Chinese hans from guangxi area around.Methods:53patients with polymyositis/dermatomyositis and100healthy controls were examined for genotypes, HLA-DQA1,-DQB1allele typing was carried out using the polymerase reaction-sequence specific primers PCR (PCR-SSP).Results:1. HLA-DQA1allele frequency distributionPM/DM patients group were detected10alleles, the gene frequency range of0.057-0.340. Among them, the DQA1*0101,*0102,*0302allele frequency was higher (0.340,0.255,0.226), DQA1*0201,*0501,*0601allele frequency was lower (respectively0.057,0.094,0.057). Control group were detected10alleles in the gene frequency range of0.015to0.260. Among them, the DQA1*0101,*0102,*0104,*0302allele frequency was higher (0.260,0.215,0.240), DQA1*0103,*0302,*0601allele frequency was lower (0.065,0.015,0.035,0.060). 2. HLA-DQB1allele frequenciesPM/DM patients group were detected five alleles, the gene frequency range of0.009to0.123. DQB1*0301allele frequency was higher (0.094), DQB1*0201,*0401frequency is low (0.047,0.019). The control group, were detected in the4alleles, gene frequency range of0.000to0.095. DQB1*0301allele frequency was higher (0.085), DQB1*0501,*0601frequency is lower (0.070,0.055).3. HLA-DQA1,-DQB1alleles frequencyPM/DM patients group and normal control group, the PM/DM patients group of HLA-DQA1*0302,*0401allele frequency was significantly increased, the difference was significant (OR values were26.759,5.244; P values were0.000correction Pc value were0.000,0.000,0.004). Alleles of HLA-DQA1*0201,*0501frequency is reduced (OR value to0.328,0.413;P values were0.018,0.028), but the correction to the Pc>0.05, not statistically significant.4. With interstitial pneumonia group, HLA-DQA1,-DQB1alleles distribution4.1PM/DM with interstitial pneumonia patient group, the HLA-DQA1were detected in9alleles, gene frequency range of0.026-0.395. DQA1*0102,*0104,*0401allele frequency was higher (0.211,0.237,0.237), DQA1*0103,*0501frequency was lower (0.079,0.079) of DQA1*0201is not seized out.4.2PM/DM with interstitial pneumonia patient group, the HLA-DQB1were detected five alleles, the gene frequency range of0.026-0.132. DQB1*0201,*0301allele frequency was higher (for the0.053,0.053), DQB1*0401,*0501frequency is lower (0.026,0.026).5. Without interstitial pneumonia group, HLA-DQA1,-DQB1alleles distribution5.1PM/DM without interstitial pneumonia patient group, the HLA-DQA1were detected in10alleles, gene frequency range from0.074to0.309, Which DQA1*0102,*0302allele frequency was higher (for the0.279,0.279), DQA1*0201,*0401frequency was lower (respectively, are0.088,0.088).5.2PM/DM without interstitial pneumonia patient group, the HLA-DQB1were detected in the4alleles, gene frequency range of0.015to0.118. DQB1*0301,*0601allele frequency was higher (for the0.118,0.118), DQB1*0201frequency was lower (0.044), DQB1*0401were not detected.6. With interstitial pneumonia group and without interstitial pneumonia group, HLA-DQA1,-DQB1alleles distributionPM/DM patients with interstitial pneumonia group alleles of HLA-DQA1*0302,*0401frequency was significantly higher than the heathy control group (OR=11.548,11.957; P values were0.003,0.000; correction Pc values were0.026,0.000). Compared with the normal control group, the PM/DM patients with interstitial pneumonia group of alleles of HLA-DQA1*0302frequency was significantly higher (OR=40.956, P=0.000, Pc=0.000), while the alleles of HLA-DQB1*0501frequency was decreased (P=0.021), but correction Pc>0.05, not statistically significant.Conclusion:Alleles of HLA-DQA1*0302,*0401have a significant genetic correlation with PM/DM, HLA-DQA1*0302,*0401with PM/DM were susceptibility genes in Guangxi Han patients with or without interstitial pneumonia may have different genetic immunological background. HLA-DQA1*0401may be dangerous gene of Han nationality in Guangxi region associated interstitial pneumonia in PM/DM patients. |
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