Analysis Of Common Chromosomal Abnormalities In Bladder Cancer By FISH Technical

BackgroundBladder cancer is one of the most common malignant tumors. its incidence rate of 1.5-3% while is also the highest mortality rate of urinary system tumors. About 70% of New bladder cancer often are superficial tumors(usually presents of Ta-is). It has high recurrence rate and fewer distant metastases. Methods of treatment for superficial bladder cancer are TURBt and laser fulguration or partial bladder resection, routinely given after intravesical instillation of local chemotherapy. For follow-up of bladder cancer patients,imageology and cystoscopy or urine cytology are regular methods, But the use of cystoscopy or imaging diagnosis makes more difficult for obvious morphological changes occur in the clinical such as flat minor lesions of the bladder tumor or bladder carcinoma in situ The cystoscopy traumatic cause trouble and suffering for many patients with bladder cancer. Urine exfoliative cytology is less sensitive to check lower for the clinical pathological grade malignant lesions.Tumor is a complex process of phased progress. Cell chromosome abnormalities found in almost all tumor lesions. Fluorescence in situ hybridization technology is based on cytogenetic-based detection technology. It uses the luciferase reagent to mark the DNA produced probes with target DNA hybrid formation can be observed in the combination. To observed this combination by fluorescence microscopy in order to detect the DNA of cells in the structure and number to diagnose bladder cancer. In bladder tumor, the most common chromosome structure and number of abnormal changes in 3,7,17 and P16 loci. Fluorescence in situ hybridization detection techniques can be detected in exfoliated urothelial cell chromosome changes.This detection technology continues to be developed and promoted. Now it is important spection methods in the preoperative diagnosis and recurrence monitoring.ObjectAnalysis of common chromosomal abnormalities in bladder cancer by FISH technical.Research MethodsAdmitted with gross hematuria or microscopic hematuria in patients with a total of 100 cases (65 cases of males and 35 females) in October 2010 to February 2011 by Department of Urology of Second Affiliated Hospital of Zhengzhou University. All patients were underwent urine FISH, urine cytology, abdominal ultrasound, urinary tract CT or intravenous pyelogram (IVP) and cystoscopy or ureteroscopy.Research Results100 cases of hematuria in patients with final diagnosis results to remove other types of diseases and detect errors, clinical imaging and pathological examination diagnosed with bladder cancer and urinary FISH-positive cases of 66 cases. The number of CSP3 distortion are 45 cases, the number CSP7 distortion are 39 cases, the number of GLP of p16 (9p21) distortion are 44 cases, the number of CSP17 distortion are 37 cases.(1) The relations of chromosomal aberrations between onset and recurrence:Distortion of GLP of p16 (9p21) in bladder cancer recurrence group (91.3%) was significantly higher than the early onset group (43.3%), the difference was statistically significant (P=0.002); Correlation between the occurrence of chromosomal deletions and recurrence.(2) The relations of chromosomal aberrations and staging of bladder cancer:Non-muscle invasive bladder cancers are 52 cases. Myometrial invasion in 14 cases of bladder cancer. Myometrial invasion in bladder cancer of GLP of p16 (9p21) and chromosome 7 aberration rate (respectively 92.8% and 92.8%) is significantly higher than the distortion of the non-muscle invasive bladder cancer (59.6%and 50%); the difference was statistically significant (P= 0.004 and 0.001, respectively); Correlation between the polyploid changes and staging.(3)Relationship between chromosomal aberrations and bladder cancer histological classification:According to histological classification, patients with bladder cancer are divided into 34 cases of non-invasive papillary urothelial carcinoma,28 cases invasive urothelial carcinoma,4 cases of urothelial carcinoma in situ. Chromosome 7, chromosome 17 and 9p21 polyploid papillary urothelial carcinoma and invasive urothelial carcinoma of the statistical analysis differences (P=0.011,0.02 and 0.003.respectively). They polyploid changes and the degree of malignancy are relevant.Conclusions1.9p21 chromosome deletion detected more frequently recurrent bladder cancer, 9p21 chromosome deletion and bladder cancer recurrence is closely related.2. Chromosome 9p21 and 7 polyploid are significantly increased in the invasive tumors and closely related with it.3.7,9p21,17chromosome of polyploidy is increased with the degree of malignancy and closely related with it.4. By detection of exfoliated cells in urine chromosome abnormalities may provide a new means of monitoring the prognosis of bladder cancer, and further clinical selection of individualized treatment of bladder cancer.