Study On The Different Levels About Arginase And Manganese In Cord Blood Of Preeclampsia Patients

Background:Hypertensive disorders complicating pregnancy severely threatens thehealth of both gravida and fetus, and much attention has been paid to theetiology and pathogenesis of preeclampsia by researchers at home and abroad.Many reports have revealed that the expression and activity of arginaseincreases in preeclampsia patients’ plasm, vasculature, and placenta. Arginaseuncouples nitric oxide synthase, reduces bodily nitric oxide composition, andincreases superoxide and peroxynitrite composition. We have also learned thatmanganese of high concentrations in cord blood raises the risk ofpreeclampsia. Meanwhile, manganese is widely believed to be an activator ofarginase. It is beneficial to study the effect of manganese in preeclampsia asan activator of arginase or an increase of its concentration.Objective:This paper studies the concentrations and activities of arginase (ARG) andthe concentrations of manganese (Mn) in cord blood samples of preeclampsiapatients and normal gravidae，and then explores the correlation between them.Method:The cord serums were collected from mild preeclampsia (n=30), severepreeclampsia (n=34), and normal pregnancies (n=32). The concentrations andactivities of arginase (ARG) were determined by using enzyme linkedimmunosorbent assay（ELISA）; the concentrations of manganese (Mn) were determined by graphite furnace atomic absorption spectrometry. The data wereanalyzed by SPSS17.0software. Then the correlation between concentrations andactivities of ARG and concentrations of manganese in cord blood samples ofpreeclampsia patients is studied.Results:(1) Concentrations and activities of arginase in the preeclampsia groupswere significantly higher than those in normal pregnancy group with the severepreeclampsia group being the highest, and these results are statisticallysignificant (P<0.01);(2) Concentrations of manganese in the preeclampsiagroups were significantly higher than those in normal pregnancy group and thisresult is statistically significant (P<0.01);(3) There is a linear positiverelationship between concentrations of manganese and activities of arginase inpreeclampsia groups’ cord serum samples, while such relationship does notexist between concentrations of manganese and arginase.Conclusion:(1) The severity of preeclampsia correlates positively with concentrationsand activities of arginase, as severe preeclampsia patients seem to exhibitsignificantly higher concentrations and activities of arginase;(2) Highconcentrations of manganese contributes importantly to disorder ofmicroelements of preeclampsia patients and thus the disease;(3) There is alinear positive relationship between concentrations of manganese and activitiesof arginase in preeclampsia patients, and the former contributes to preeclampsiaadditionally by increasing activities of arginase.