Studies On Extraction, Purification And Activity Of Total Tannins In Sanguisorba Officinalis L.

Sanguisorba officinalis L. is the perennial herb for the Sanguisorba L. of Rosaceae.In the northeast of China, Sanguisorba L. mainly includes S. officinalis L., S. officinalic Lvar longifalia (Bert) Yu et Li, S.grandifolia（Maxim）Makino, S. sitchensis C.A.Mey, S.tenuifolia Fisch. ex Link, S. parriflora (Maxim.) Takeda and S. officinalis L. var. carnea(Fisch.) Regel ex Maxim. Sanguisorba Radix is the dried roots of Sanguisorba officinalisL., and first recorded in“Shen Nong’s Herbal Classic” as one of the well-known Chinesetraditional medicines. Sanguisorba Radix is widely used for the treatment of the bleeding,the fire burns, typhoid fever, etc.At present, the studies of tannins have become a most active field in the naturalpharmaceutical chemistry. In order to develop and make use of this Chinese traditionalmedicine better and provide scientific data for the traditional Chinese medicinemodernization, we have studied the tannins and pharmacological activities of the roots ofSanguisorba officinalis L. in this paper.1. Referring to the related literatures, we reviewed the latest research progress ofSanguisorba officinalis L. in several aspects such as plant morphology, chemicalconstituents, pharmacological activity of Sanguisorba officinalis L. and tannins.2. In this paper, we select the UV spectrophotometric method that determined thecontent of total tannin of Sanguisorba Radix. The method is using gallic acid as referenceto measure absorbance and draw standard curve and using phosphomolybdic tungstic acid–casein to calculate the content of tannin. We chose several factors that may influenceresults of extraction, including concentration of ethanol, volume, time and times ofextraction. First, we did single factor experiments to determinate the extent of these factors,and then orthogonal test showed that the best technology is reflux extraction with70%ehanol (15folds) for3times, an hour once. Validation experiments were also done to makesure the technology stable applicable.We purifica the tannins of Sanguisorba Radix by adopting method of gelatinprecipitation and being extracted with ethyl acetate. The results show that content oftannins of Sanguisorba Radix is greater than73%. 3. According to acute toxicity testing of total tannins of Sanguisorba Radix, themaximum administered dose is10g/kg in mice and we can not test the value of LD50.According to acute gastric ulcer in mice and observe the ulcer stuation of gastric gland, themedium and high dose group of total tannins of Sanguisorba Radix can inhibit gastric ulcerof the mice with the method of group T test. By researching, the total tannins ofSanguisorba Radix also can effectively reduce bleeding and clotting time. It’s showing thattotal tannins of Sanguisorba Radix have a better hemostasis.