Effects Of Nerve Growth Factor Combination With Insulin On Wound Healing With Ⅱ Degree Burn And The Regneration Of Epidermis Cells In Diabetic Rats

Objective To investigate the effects of local application of NGF combine with insulin on wound healing with II degree deep scald and the expression of skin stem cells markers β1integrin and keratin19in diabetic rats. Methods60male Wistar rats were used in the study by intraperitoneal injection of streptozotocin. Deep partial thickness scalding was engendered on the back of the rats after one month. Then these rats were randomly divided into B (diabetes control,n=15), C (insulin treatment, n=15), D (NGF treatment, n=15) and E (NGF and insulin treatment,n=15)groups, and group A (nomal control,n=15) was created by normal rat of partial thickness scalding.The wound area, wound healing rate, and express of skin stem cells markers β1integrin and keratin19(K19) immunohistochemistry staining were investigated after3,7,15, and21day post scalding days (PSDs).Results Compared to those in groups A、B、C and D, the wound healing rate in group E (25.33±2.32)%increased significantly since the7th PSD (P<0.05). The wound healing rate in group A C and D increased significantly compare with group B [(22.51±1.78)%,(16.68±1.95)%,(18.29±1.70)%,(P<0.05)]. The expression of β1integrin and keratin19(K19) in group E increased significantly compare with other groups from7th day till21st day(P<0.05). Conclusion Local application of NGF combine with Insulin could be beneficial to promote skin stem cell proliferation and differentiation in the burn wounds of the rats with diabetes and accelerate wound healing.