Studies On The Chemicial Constituents Of Premna Serratifolia L And Starfish(zdldw032)

Two parts are involved in this dissertation : The studies on the chemical constitu- ents of Premna serratifolia L and The studies on the chemical constituents of Starfish (ZDLDW032). These two commonly used Chinese herbal medicines were isolated by modern chromatographic methods including CC, PTLC, ODS, Sephadex LH-20 and HPLC, and twenty-three compounds were gained. The structures of Nineteen compounds were elucidated by spectroscopic methods including NMR , IR and MS.Premna serratifolia L, which belongs to the family of Verbenaceae, commonly used to treat people bitten by snakes, post-traumatic bleeding, pain, bruises, toothache, burn , malaria, dysentery etc. Eighteen compounds were isolated from the Premna serratifolia L and Fifteen compounds in them were identified as:piscrocins D(1), rehmaglutinA(2),10-O-(Z)-p-coumaroylcataipol(3), 10-O-(Z)-p-coumaroylcataipol(4),β-ecdysone(5), Integrister-oneA20,22-acetonide(6),β-sitosterol(7),β-daucosterol(8), Genkwanin-5-O-β-D-glucopyranoside(9),Quercetin(10),Kaempferol-3-O-β-D-galact-opyranoside(11),Apigenin(12),3′,5,7-trihydroxy-4-methoxylflavonoid(13),2-hexylidene-3-methyls-ccinic acid(14), thymine-2-desoxyriboside(15).Starfish (phylum Echinodermata, class Asteroidea), which is one of the common large invertebrate, have proven to be an especially valuable source. The investigation of active component and mechanism of action of Starfish draws more and more scholars. At present, many polytype compounds have already been discovered. Starfish contains different types of bioactive materials, such as proteins enzymes, steroidal glycosides, gangliosides, alkaloids etc. These compounds have activity of anticancer, antioncoma, bacterio-stasis, ichthyotoxin, antioxygen and so on. Six compounds were isolated from the Starfish(ZDLDW032) and Five compounds were identified as:amu- rensoside D(16), cholesterol(17), Ergosta-5,24(28)-dien-3β-ol (18), batyl alcohol(19).