Establishment Of Rat Orthotopic Liver Transplantation And Analysis Of Reasons For Failure

Objective：The most commonly used animal model of livertransplantation is rat orthotopic liver transplantation (ROLT) and“two-cuff”method is the standard technique. It is difficult and complex to establish“two-cuff”method of ROLT. The purpose of this study is to learn how toestablish this model, to analyze and summarize technical difficulties of themodel, to practice the microsurgical operation technology and build a goodplatform for liver transplantation experimental study.Methods：One hundred fifty cases of ROLT were conducted by modifiedKamada“two-cuff”method and the learning process was divided into threestages: early stage, middle stage and late stage. To analysis the operation timeof donor and acceptor, time of an-hepatic phase, success rate of operation,survival time of post-transplantation, survival rate and reasons for failures indifferent stage.Results：There have 0 case, 8 cases and 21 cases models weresuccessfully made by“two-cuff”method respectively in early, middle and latestage, and the total success rate was 19.3%. The time of donor operation,receptor operation and an-hepatic phase in early stage was obviously longerthan middle stage and late stage with statistical significance (P<0.05); thetime of donor liver trim was gradually shortened, and there was no statisticalsignificance. The main reasons for failures in ROLT included: anesthesia deep,pneumothorax, blood loss, bad donor lavage, failure of donor trim, and longeran-hepatic phase, failed of anastomosis of suprahepatic inferior vena cava (SHIVC), portal vein and infrahepatic inferior vena cava (IHIVC), and failedof bile duct reconstruction. The main reasons for postoperative death included:(1) postoperative bleeding; (2) thrombosis or embolism; (3) graft nonfunction;(4) postoperative infection; (5) biliary obstruction and bile leakage.Conclusions：“Two-cuff”technique of ROLT model was relativelycomplicated with its technical difficulties, many factors could lead to failure.To analyze the common reasons for failures in ROLT and summarize thecorresponding solutions in time would be helpful to the beginners, and whocan master this technique in a patient and meticulous way after a period oftraining.