Experimental Research On Auxiliary Partial Orthotopic Liver Transplantation For Acute Liver Failure

Experiment Ⅰ Establishment of acute liver failure model in miceObjective To establish an acute liver failure model in mice using major hepatectomy combined with ischemia injury which is suitable for surgery research.Methods Male C57BL mouse was performed82%of whole liver resection combined with25min total hepatic vein exclusion under consistently inhalation of oxygen/isoflurane.Results native liver histology showed significant lymphocyte and neutrophil infiltration6h and massive hydropic degeneration with severe hepatic lobule structure disorder at24h after major hepatectomy and ischemia reperfusion injury.Conclusions An acute liver failure using hepatectomy combined with ischemia injury was established for the first time which was appropriate for liver injury and APOLT research. Experiment Ⅱ The therapy of mice acute liver failure using auxiliary partial orthotopic liver transplantationObjective. Auxiliary partial orthotopic liver transplantation (APOLT) is an effective treatment for fulminant liver failure. However, information on the experimental problem and hepatocyte proliferation mechanism of native and donor liver is lacking. In this study, an effective APOLT model for acute liver failure (ALF) in mice was established to address these issues.Method. We created an ALF mouse model wherein about82%of liver tissue was resected with total hepatic vascular exclusion. The procedure lasted25min. Donor liver was transplanted to the left lateral lobe of the C57BL of the recipient.Results. In the ALF group, most mice died within2d because of liver failure. Of the33mice that received APOLT,27lived for more than7d. Significantly increased the levels of transaminases. serum ammonia, total bilirubin and liver cell apoptosis were observed in the ALF group, whereas treatment with APOLT reduced all these parameters. In addition, significant regeneration of the native liver with the auxiliary liver graft was observed in the APOLT group.Conclusions. It was proved that APOLT could totally meliorate the liver functions, reduce the native liver apoptosis, and facilitate the native liver proliferation in acute liver failure by means of mouse APOLT model which was established for the first time.