Isolation And Structure Identification Of Natural Glycopeptides

In order to investigate the chemical structures of glycopeptide, the isolation and purification, structural elucidation were performed on the glycopeptides A. Two homogeneous glycopeptides(B1 and B2) were obtained by DEAE-cellulose and gel filtration chromatography. Their structure of B1 and B2 were identified by a variety of methods including HPLC, GC-MS, methylation analysis,13CNMR and IR.The results were as follows:B1 consisted of mannose and glucose with a molecular weight of 5.69×104. The molar ratio of mannose and glucose was 1:0.05 and the chain of B1 included terminal,1,2-linked, 1,4-linked,1,6-linked,1,2,6-linked,1,2,4,6-linked Man and 1,4-linked Glc.B1 had16 amino acids. The total content of amino acids was 3.88g/100g and the connection of polysaccharides and peptides was O-glycosidic linkage.B2 consisted of mannose and glucose with a molecular weight of 1.55×104. The molar ratio of mannose and glucose was 1:0.05 and the chain of B2 included terminal,1,2-linked, 1,4-linked,1,6-linked,1,2,6-linked,1,2,4,6-linked Man and 1,4-linked,1,3,4-linked,1,2,4-linked Glc. B2 had16 amino acids. The total content of amino acids was 6.15g/100g and the connection of polysaccharides and peptides was O-glycosidic linkage.