Study On Isolation, Purification, Structure Identification And Elementary Biological Activities Of Polysaccharides From Two Kinds Of Plant

Talinum paniculatum Gaertn. and Paederia scandens (Lour.) Merr. were separately extracted by ethanol and hot water successively. The water extract were deprotinized by Sevage method, precipitated by ethanol, and then the crude polysaccharides were obtained. The crude polysaccharides were isolated and purified by DEAE-cellulose ion-exchange column chromatography and Sepharose CL-6B gel column chromatography, and then the homogeneous polysaccharides were gained. The purity of homogeneous polysaccharide was identified by gel column chromatography and polyacrylamide disc gel electrophoresis, and molecular weight was identified by GPC. Their structure was analyzed by GC, IR, UV and PC. The biological activities of homogeneous polysaccharide were elementarily investigated. Finally, five kinds of homogeneous polysaccharides were obtained from the two kinds of plant. So far, the five kinds of homogeneous polysaccharides and their biological activities have not been reported yet, and the related results as follows.Two kinds of homogeneous polysaccharides, TPP3a and TPP5b, were obtained from Talinum paniculatum Gaertn.. TPP3a was an acidic polysaccharide, consisted of rhamnose, arabinose and galactose in the molar ratio of 0.81:1.04:1.00. Its saccharide and uronic acid content were 79.04% and 22.66% respectively. TPP3a mainly contained pyranose and linked byβ-glycosidic bond in its backbone. The number-average molecular weight (Mn) and weight-average molecular weight (Mw) of TPP3a were 5.275×104, 1.076×105 respectively. TPP5b was a neutral heteropolysaccharides, consisted of mannose and glucose in the molar ratio of 1.00:4.50. Its number-average molecular weight (Mn) and weight-average molecular weight (Mw) were 7.682×103, 8.452×103 respectively. TPP5b mainly contained furanose and its saccharide content was 73.74%.Under the model of induction of PC12 cell differentiation, NGF as positive control, TPP5b (20μg/mL) could induct PC12 cell differentiation in vitro, but TPP3a couldn't. Three kinds of homogeneous polysaccharides, PSP2a, PSP2b and PSP3a, were obtained from Paederia scandens (Lour.) Merr.. PSP2a, an acidic polysaccharide, mainly contained furanose and consisted of arabinose, galactose and glucose in the molar ratio of 0.81:1.04:1.00. Its saccharide and uronic acid content were 69.12% and 3.62% respectively. PSP2b, an acidic polysaccharide, mainly contained furanose and...