The Response Of Metabolic And Physiological Characteristics Of C. Chinensis To Enhanced UV-B Radiation

In order to elucidate the regulation mechanism of different intensity of UV-B on the metabolism of the C. chinensis body berberine and C. chinensis physiological response to UV-B. The experimental set four treatment groups:CK (natural light), UL (0.05 W·m-2), UM (0.10 W·m-2), UH (0.20 W·m-2) to explore long-term (35d daily radiation 3h) UV-B radiation C. chinensis primary metabolism in the photosynthetic characteristics of PPP pathways and secondary metabolites in tyrosinase activity and C. chinensis root, stem, leaf content of berberine change characteristics; to explore short-term (1-9h) UV-B in characteristics of POD, SOD, CAT, isozyme, Stress Indexes of C. chinensis, as well as using UV-B (0-30min) to deal with C. chinensis leaves, the use of [y-P32] ATP isotope is to test activity characteristic of protein kinase. Results are as follows:1. Kinase activity activated transiently, the radiation intensity of y-P32 by liquid scintillation counter. UL, UM radiation processing C. chinensis leaves for I min, the kinase activity was significantly enhanced, and the UL group kinase activity is greater than the UM group, the kinase activity began to decline after the intensity of UL radiation for 2 min, and to a minimum level after 30 min, the UM intensity radiation 1min after the kinase activity began to fall, basically returned to normal levels after 5 min. However, kinase activity of C. chinensis leaves stay balance under severe UV-B radiation.2. Photosynthetic pigments in the C. chinensis leaves, qN, the Fo ETR and the glucose-6-phosphate dehydrogenase activity, root of berberine content is lower than other groups under UH radiation condition. However, photosynthetic capacity, glucose-6-phosphate dehydrogenase activity, tyrosinase activity and the content of berberine in roots were higher than the control groups under UM radiation condition, the phenomenon may be due to the fact that C. chinensis may produce stress response against the light and moderate UV-B radiation.3. UV-B radiation after 3h, the SOD1 (Rf=0.125), the SOD2 (Rf=0.312), CAT1 (Rf= 0.428), POD3 (Rf=0.290), POD4 (Rf=0.636) band was successively induced, with the time the growth of UV-B radiation, and these bands began to be lost or weakened, especially in severe UV-B stress, CAT1 (Rf=0.428), POD3 (Rf=0.290) bands will be lost in advance; In addition, UV-B radiation makes the leaves of MDA, soluble sugar, proline content was significantly higher than the control, UV-B radiation 7h, in addition to the UL group of proline increased in each group of MDA, soluble sugar, proline content began to decline in the number of soluble protein bands increased.4. Accumulation of Berberine in C. chinensis leaves happens under UL radiation processing 35d condition, moreover, handle 21 d is conducive to the accumulation of berberine in the root, with an increase of 46.9%; the UM radiation treatment C. chinensis 14d, Berberine Content of the leaves of C. chinensis with the rapid accumulation, increase comes up to 72.9%,35d there is conducive to the accumulation of the content of berberine in the root; UH radiation processing C. chinensis more than 21d will lead to decreased berberine content in the roots and leaves, but the content of berberine in the stem show the increase trend.Conclusion:UL (mild), UM (moderate) radiation are beneficial to induce the accumulation of berberine content in roots and leaves, probably due to C. chinensis in the UL, the UM radiation, the increased kinase activity, activation of the related enzyme activity and gene expression, enhanced photosynthesis and PPP pathway, resulting in more secondary metabolites precursors and secondary metabolites required NADPH, and then the content of berberine also increased accordingly. Furthermore, the radiation intensity induced C. chinensis increased antioxidant isoenzyme expression and the number of bands, resistance to the accumulation of soluble sugar, soluble protein substances to improve their resistance ability in UL, UM group. Although the UH (severe) of UV-B radiation can make the content of berberine accumulated in the stem, the content of berberine of the root and leaves of C. chinensis reduced, the reason probably is that severe UV-B radiation long duration will destroy its own regulate conditioning systems, furthermore, metabolism began to disorder, berberine synthesis blocked.