The Significance Between Telomerase Activity And High Risk Human Papillpmaviru Load In Cervical Lesions

Objective To investigate the function and clinical significance of high risk human papillomavirus(HR-HPV) infection and telomerase in the development of cervical carcinoma, the HR-HPV and telomerase were detected in cervical lesions. Methods HPV-DNA in cervical exfoliated cells was detected by second-generation hybird capture technology, then the expression of telomerase was evaluated by immunohistochemistry. Results (1) The positive rates of telomerase in control group, cervical intraepithelial neoplasia(CIN) Ⅰ group, CIN Ⅱ group, CINⅢ group and cervical carcinoma group were10.00%,16.67%,40.00%,70.00%,95.00%, respectively. The positive rate of telomerase in cervical cancer was significantly higher than that in CINⅢ (x2=4.329, P=0.037). And the positive rate in CINⅢ was significantly higher than that in CIN Ⅱ (x2=4.327, P=0.038). And the positive rate in CIN Ⅱ was significantly higher than that in CIN Ⅰ (x2=4.022, P=0.045).(2) With the deterioration of carcinoma lesions, HR-HPV load and the positive rate of HPV infection increased gradually. The positive rates of HPV infection were significantly different among the cervical cancer group, CINⅢ group and CIN Ⅱ group, CIN Ⅰ group and control group (x2=7.414-29.501, P<0.01). The high risk HPV load was significantly different between CIN Ⅰ group and CIN Ⅱ group, CINⅢ group, cervical cancer group (P<0.05).(3) The positive rates of telomerase in each group of HPV positive were0%(0/5)、25.00%(2/8)、37.50%(6/16)、72.22%(13/18)、100.00%(19/19), respectively. There were significantly different among CINⅢ、cervical cancer group and the other groups (P<0.05). Conclusion There was obvious correlation between high-risk type HPV infection and the expression of telomerase in cervical intraepithelial neoplasia and cervical cancer, which might be used markers to monitor and predicate the transit of early cervical cancer in clinical practice.