The Influence Of Magnesium Supplementation On The Infinity Of Insulin Receptor Of Rats With Type2Diebetes Mellitus

Objective:To observe the influence of magnesium supplementary to rats with type2diabetes on glucose metabolism and insulin receptor affinity.Methods:Take male Wistar rats60as experimental subjects,adaptable feeding for one week.Then selected10rats randomly as normal control group,the remaining50fedwith high-fat diet combined with low dose intraperitoneal injection of streptozotocin to indeduced type2diabetes rats model.Time for model induced is five weeks.Then the diabetes rats were randomly divided into four groups:diabetic control group,high,medium and low dose magnesium supplementation group.Adding different doses of magnesium2000,1000,200mg/kg to high, medium, low dose groups.All rats continued to be fed for4weeks,each weekend fasting tail tip blood glucose were measured for1time.Magnesium supplementation for four weeks,then all animals were anesthetized,take the abdominal aortic blood,using radioimmunoassay to test fasting serum insulin levels,using radioactive receptor assay to test liver cells insulin receptor affinity and binding capacity,use colorimetric detection to test glycated hemoglobin and serum malondialdehyde content.Results:(1)Fasting blood glucose levels after magnesium supplementation:magnesium supplementation after1week,2weeks,there is no significant difference between3magnesium supplement group and diabetic control group (F=0.19,0.63,P>0.05);the3th week:3magnesium supplementation groups compared with the diabetic control group,the difference was statistically significant(F=3.23,P<0.05),between groups comparison:high dose group,fasting plasma glucose vas (17.70±0.49) mmol/L, diabetic control group was (18.75±0.89) mmol/L,the difference was statistically significant (q=4.33,P<0.05);the4th week:3Magnesium supplementation group compared with the diabetic control group, the difference was statistically significant (F=3.60,P<0.05),between groups comparison: fasting plasma glucose in high dose group was (17.45±0.63) mmol/L, the diabetic control group was (18.55±0.96) mmol/L,fasting plasma glucose in high dose group was decreased compared with diabetic control group, the difference was statistically significant (q=4.63,P<0.05).(2)Fasting serum insulin:at the end of the experiment, insulin levels of3magnesium supplemented group were (18.95±0.60)、(19.34±0.54)、(19.39±0.60) μIU/mL,the diabetic control group was (19.95±0.44) μIU/mL,the difference between groups was statistically significant (F=5.67,P<0.05).Between each two groups:high,medium and low dose group, insulin levels compared with diabetic control group was reduced, the difference was statistically significant (q=5.778,3.545,3.256,P<0.05).High, medium and low dose group showed no significant difference (P>0.05).(3)Insulin resistance index:at the end of the experiment,insulin resistance index in high, medium,low dose group were:14.60±0.53,15.57±0.60,15.56±0.73,diabetic control group was16.41±0.65,the difference between group was statistically significant (F=13.80,P<0.05).Between any two groups:high, medium and low dose group and diabetic control group,the difference was statistically significant (q=9.09,4.23,4.22,P<0.05).High, medium and low dose group showed no significant difference (P>0.05) between each group.(4)Insulin sensitivity index:by the end of the experiment, insulin sensitivity index in high,medium and low dose group were:-5.79±0.04,-5.87±0.04,-5.89±0.07,diabetic control group was-5.90±0.04,the differences between groups statistically significant (F=9.86,P<0.05).Between any two groups:the sensitive index of the high dose group compared with the diabetic control group increased,the difference was statistically significant (q=7.12,P <0.05);the sensitive index of the medium and low dose group and the diabetic control group was no significant difference (P>0.05); sensitive index of the high dose group increased than in the low dose group, the difference was statistically significant (q=4.79,6.05,P<0.05).(5)Insulin receptor affinity:receptors is divided into two kinds:high affinity receptor and low affinity receptor, K1represent affinity of the high affinity receptor, K2represent affinity of the low affinity receptor. At the end of the experiment, the three dose groups K1,K2and diabetic control group, the difference was statistically significant (F=4.17,3.95, P<0.05);between any two groups:high dose group K1(4.76±0.08)×108L/mol, and the diabetic control group (4.57±0.09)×108L/mol,and the high dose group K1compared with diabetic control group increased,the difference was statistically significant (q=5.00,P<0.05);high-dose group K2is (1.10±0.14)×106L/mol,and the diabetic control group (0.87±0.10)×106L/mol was increased compared with the diabetic control group, the difference was statistically significant (q=4.86,P<0.05).(6)Insulin receptor binding capacity: ①The high affinity receptor binding capacity R:the difference between3dose group and the diabetic control group was no statistically significant (F=1.549, P>0.05).②The low affinity receptor binding capacity R2:the level of R2in3dosage group, respectively (8.49±0.43),(7.82±0.37),(7.72±0.50)x1013/mg protein,diabetic control group (7.62±0.45)×1015/mg protein, difference between group was statistically significant (F=7.86, P<0.05);between any two groups:R2in high dose group increased compared with diabetic control group,the difference was statistically significant (q=6.19,P<0.05).High dose group R2increased compared with the low dose group, the difference was statistically significant (q=4.79,5.49,P<0.05).(7)Glycosylated hemoglobin:by the end of the experiment, high, medium and low dose group, glycated hemoglobin levels were26.75±3.59,29.46±8.11,3.05±6.96,diabetic control group was33.77±5.17, the difference was statistically significant (F=7.33.P<0.05). Between any two groups:the high dose group were decreased compared with the diabetic control group, the difference was statistically significant (q=2.85,P<0.05).(8)Serum malondialdehyde content:by the end of the experiment, MDA content of the high, medium and low dose group were (5.61±0.37)、(5.98±0.48)、(6.03±0.58) nmol/ml respectively,of the diabetic control group was (6.44±0.38) nmol/ml, there was statistically significant difference (F=3.32, P<0.05).Between any two groups compared with the high dose group compared with the diabetic control group decreased, the difference was statistically significant (q=4.46,p<0.05).Conclusions:The magnesium supplement can reduce fasting blood glucose and serum insulin levels of type2diabetic rats, reduce insulin resistance index of type2diabetic rats,increase the insulin sensitivity index, increase insulin receptor affinity and low affinity insulin receptor binding capacity, and improve insulin resistance status and reduce the level of lipid peroxidation.