Progression Of Cervical Lesions And Its Association With The Human Papillomavirus And The Telomerase RNA Components

Background and Objective:Cervical cancer as one of the three common gynecologic malignancy, is a serious threat to the health and lives of women. With the rapid development of science and technology in recent years, a number of factors with cervical cancer occurrence and development are also to be recognized. Hausen’s study found that human papillomavirus (HPV) is a necessary cause of cervical cancer. Another study found that telomeres and telomerase is closely related to the occurrence and development of a variety of tumors, including cervical cancer. Increased expression of the telomerase RNA component (TERC gene) can be found in precancerous lesions of cervical cancer and cervical cancer cell lines. Cervical cancer incidence and mortality has been gradual decline in recent years, in a sense it depends on early detection of relevant factors like HPV infection and TERC gene amplification and so on in cervical lesions, and reasonable and effective early intervention.To detect human papillomavirus infection and telomerase RNA component (TERC) amplification in the progression of cervical lesions, and assessment the significance of HPV testing, the TERC gene detection in early diagnosis of cervical cancer.Methods: 1. comply with the conditions in the screening of88patients according to their histopathologic findings groups:10cases of normal group,25cases of CIN I group,37cases of CIN Ⅱ-Ⅲ group,16cases of cervical cancer (including periods) group.2. using gene Hybrid-2Capture (HC-2) to detect HPV infection,3. using fluorescence in situ hybridization (FISH) to detect the TERC gene expression,4. using the SPSS13.0software to analyze the data obtained, the rate was analyzed by χ-test, correlation analysis using Pearson correlation, r was the correlation coefficient, a=0.05for the inspection level, P<0.05represent that there were statistically significant.Results:1. By HC-2method to detect HPV infection, obtained with the cervical pathology level increased, the positive rate of HPV infection is higher. The difference of HPV infection positive rate in each group was statistically significant (P=0.000). And in addition to the normal group and the CIN I group the difference of HPV infection positive rate was not statistically difference (P=0.179>0.05), the difference between the remaining two each were statistically significant (P<0.05).2. By FISH to detect the TERC gene expression results, obtained with the cervical pathology level increased, the positive rate of TERC gene amplification is higher. The difference of TERC gene amplification positive rate in each group was statistically significant (P=0.000). And in addition to the normal group and the CIN I group the difference of TERC gene amplification positive rate was not statistically difference (P=0.179>0.05), the difference between the remaining two each were statistically significant (P<0.05).3. The positive rate of TERC gene amplification and HPV infection were not equal, the difference was statistically significant (P<0.05). There were positive correlation between the two, and the correlation coefficient was0.41.4. HPV detection and TERC detection have different sensitivity and specificity: the HPV detection sensitivity:85.25%, specificity:90%. TERC gene detection sensitivity:83.87%, specificity:100%.Conclusion:1. HPV infection and TERC gene amplification is closely related to the cervical lesion progression, it can be speculated that the detection of HPV and TERC as a clinical screening of cervical lesions and disease monitoring have great significance in the diagnosis of early cervical cancer.2. HPV detection and TERC gene detection complement each other used in clinical screening for cervical cancer, that has a certain significance.