Expression Of PLCE1in Lung Carcinoma Cells And Its Clinical Significance

1BackgroundLung cancer is common malignancy, the death of lung cancer patients occupy a primary position in all cancer. The incidence and mortality of lung cancer in industrialized countries is significantly higher than the industrial developing countries. The incidence of cnacer type of China, lung cancer in male occupies the first and the female ranks after breast cancer. Lung cancer have seriously affect human health and lift, with experts and scholars’great efforts, the achievements have been made in lung cancer curation and prevention, the survival rate and quality of life has been significantly improved. It is estimated that the five-year survival rate of patients with lung cancer has increased15%. Currently, the main treatment of lung cancer is resection, chemotherapy, radiotherapy and targeted therapy, but the result of treatment is not satisfied, the five-year survival rate is still in low level. Many factors affect the treatment, the malignant biological behavior is the main factor affect the clinical treatment and prognostic of lung cancer. When most patients visit the patients, they already in the later period of the lung cancer, there maybe local invasion or distant lymph node and visceral metastasis, some patients with lung cancer is also associated with serious complications, early diagnosis and treatment may contribute to decrease the mortality rate of lung cancer. Many experts and scholars are pursuing the early diagnosis of lung cnacer. Currently, experts and scholars have found EGFR gene affect the lung cancer,but still not found the key gene to determine the therapy and prognosis of lung cancer.Wang LD et al. found PLCE1gene is closely related to the high risk of esophageal cancer, the expression of PLCE1was increased with the disease become much more serious. Esophageal squamous cell carcinoma is the most common histological type (above95%), recent studies suggest that esophageal squamous cell carcinoma and lung squamous cell carcinoma have similar molecular mechanisms. Phospholipase C epsilon-1(PLCE1) gene is discovered in recent years, it is a new member of phospholipase C (PLC) family, also found it was a new PLC isozyme. The molecular structure is complicated, it is a key enzyme in phosphatidylinositol signal transduction pathway, its plays an important role in the cell membrane transduce to the nucleus, it is involved in cell signaling, regulation of gene expression and regulate the cell growth, differentiation, participate in the process of apoptosis and angiogenesis.In this study, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) method were use to detect the expression of PLCE1in lung squamous cell carcinoma and its relationship with clinicopathological features, analysis the biology of lung cancer, explore the function and significance of the PLCE1gene in lung squamous cell carcinoma.2Materials and Methods2.1Immunohistochemical analysis2.1.1PatientsThis study enrolled96surgically resected lung tissue samples, including48cases lung squamous cell carcinoma,48cases adjacent normal tissues. The male have44cases and4cases female, the mean age is59.34. All cases have not preoperative radiotherapy, chemotherapy and other treatment.The antibody for PLCE1was buy form American Sigma company, it is polyclonal rabbit anti-human. EnVision kits were bought from DAKO Company. Every batch had control and negative groups.2.1.2Statistical MethodsThe data were analyzed by SPSS17.0. The x2test was applied for different groups comparison (a=0.05).2.2RT-PCR analysis2.2.1PatientsOne-hundred surgically resected tissue samples were enrolled in this study, including50cases lung squamous cell carcinoma,50cases normal controls. All cases have not preoperative radiotherapy, chemotherapy and other treatment.2.2.2MethodsDetecting the expression of PLCE1gene in50cases lung squamous cell carcinoma and50cases adjacent normal lung tissue with RT-PCR, and analysis the gene expression combine with the clinical pathological characteristics and features.2.2.3Statistical MethodsThe data were analyzed by SPSS17.0software. The t test, t’ test, one-way ANOVA and LSD-t test were applied for different groups comparison (a=0.05).3Results3.1Expression of PLCE1in lung squamous cell carcinomaThe immunohistochemistry positive rates of PLCE1expression in lung squamous cell carcinoma were83%(39/48), higher than the normal controls (6/48,12.5%)(P<0.01). The expression of PLCE1has relationship with lymph node metastasis and tumor staging (P＜0.01), non-relationship with age, sex and tumor differentiation (P>0.05).3.2RT-PCR in detecting the expression of PLCE1The expression of PLCE1mRNA in cancer tissues was higher than adjacent normal lung tissues (P<0.01). TNM staging of lung squamous cell carcinoma was related with the tumor histological classification, lymph node metastasis and tumor staging (P<0.05), non-relationship with sex, age (P>0.05).4Conclusion4.1Immunohistochemistry results suggest:PLCE1may play an important role in the development of lung squamous cell carcinoma.4.2The detection of PLCE1may contribute to prognosis lung squamous cell carcinoma.