The Expression Of HTERTmRNA In Peripheral Blood Of Patients With Early Stage Endometrial Carcinoma During Perioperative Period And Its Clinical Significance

ObjectiveTo detect the expression of human telomerase reversetranscriptase (hTERT) in peripheral blood of patients with endometrialcarcinoma during perioperative period and explore the feasibility ofhTERT as a molecular marker for monitoring the micrometastasis ofpatients with endometrial carcinoma.The purpose of this study is also toanalyze the influence of surgical operations on micrometastasis inperipheral blood of patients with endometrial carcinoma.MethodsFluorescent quantitative reverse transcription polymerase chainreaction(FQ-PCR)was used to measure the mRNA of hTERT inperipheral blood of patients with20cases of normal controls,20casesof uterine benign disease and28cases of endometrial carcinoma. Andcomparative analysis of the expression of hTERT mRNA in peripheralblood of patients with endometrial carcinoma during perioperativeperiod. All datas were analyzed by SPSS17.0statistic software.Results1. The positive rate of hTERTmRNA in peripheral blood ofendometrial carcinoma was57.1%(16/28),and it was higher than thatof normal controls0.0%(0/20) and uterine benign disease5.0%(1/20), with statistically significant difference (P<0.05).2. The content of hTERTmRNA of16cases which positiveexpression are relatively content with FIGO stage. The relative amountof hTERTmRNA with FQ-PCR method inⅠ~Ⅱ stage andⅢ~Ⅳ stagewere-4.0000±0.2201and-2.7107±0.1253respectively,and the relativeamount of Ⅲ~Ⅳ stage was higher than that ofⅠ~Ⅱ stage (P＜0.05).3. The peripheral blood samples from28endometrial carcinomapatients undergoing radical resection were collected before surgery andimmediately after surgery. The positive rate of hTERTmRNA in this twogroups were57.1%(16/28)and85.7%(24/28).The expression of hTERTmRNA in the surgery group was significantly higher than that inpreoperative group (P＜0.05).ConclusionsMicrometastasis can occur in the early stage of endometrialcarcinoma after surgery and the detection of hTERTmRNA in peripheralblood may contribute to the early diagnosis of micrometastasis of thesepatients and also guide the individualized treatment.