Expressions And Significance Of Myocardin And NF-κB In Uterine Leiomyoma

Objective:Uterine leiomyoma is the most common female reproductive tract tumors, but its exact pathogenesis is not clear. The pathological changes of uterine leiomyoma caused by excessive estrogen and progesterone, conventional endocrine therapy made uterine leiomyoma stop growing or shrink, but may recur after stopping. It became a hotspot that the pathogenesis of uterine leiomyoma was studied and prevented. Recent studies about the differentiation of smooth muscle cells may bring break on the molecular mechanism of uterine leiomyoma. Recently myocardin was discovered as transcriptional cofactor of serum response factor and it promoted differentiation of smooth muscle cells and can selectively activate the smooth muscle-specific gene transcription. Recent animal experiments showed that nuclear factor-KB inhibited myocardin-induced smooth muscle cells differentiation, so that the smooth muscle cells lost their normal contractile properties. Is it true that NF-κB inhibit myocardin-induced smooth muscle cells differentiation in uterine leiomyoma?This study investigated to compare the expression of myocardin and NF-κB in normal myometrium, uterine leiomyoma tissue and primary cells, and explore the relationship between these molecules and pathogenesis of uterine leiomyoma.Methods:In this study, formaldehyde-fixed, paraffin-embedded specimen from81patients with uterine leiomyoma (56cases of typical,25cases of highly cellular leiomyomas) and normal myometrium adjacent to the tumor greater than2cm were obtained from the First Affiliated Hospital of Shanxi Medical University.81cases did not receive hormone therapy preoperative at least three months, aged28-54years, mean age44.6years, pathological diagnosis of uterine leiomyoma. The expression of myocardin and NF-κB was detected by immunohistochemical staining.25cases were cultivated by collagenase digestive method and detected the expression of myocardin and NF-κB by immunocytochemistry staining. Statistical analysis was performed using the SPSS13.0software package.Results:1. The expression of myocardin in uterine leiomyoma tissue was significantly lower than normal myometrium (P<0.05), but not related to age, number of leiomyoma or degeneration.2. The expression of NF-κB in uterine leiomyoma tissues was higher than normal myometrium (P<0.05), but not related to age, number of leiomyoma or degeneration. The expression of two protein was correlated with each other (P<0.05).3. The use of collagenase digestive method can obtain a large number of primary uterine leiomyoma cells, and the abnormal expression of myocardin and NF-κB in primary cells were consistent with the counterpart tissue immunohistochemistry. Conclusion:1. Abnormal expression of myocardin is associated with the development and progression of uterine leiomyoma.2. The high expression of NF-κB is related to the development and progression of uterine leiomyoma. NF-κB may interact with myocardin, then leads to tumor occurrence.3. Uterine leiomyoma cells was obtained successfully by collagenase digestive method, and it was the consistency with the expression of myocardin and NF-κB in paraffin-embedded tissue, in order to further study the protein function in uterine leiomyoma.