The Expression Of Cyr61 And Basic-fibroblast Growth Factor In The Uterine Leiomyoma

Objective:Set up experimental model of human uterine leiomyoma cells/smooth muscle cells in vitro,to determine the levels of Cyr61 and basic-fibroblast growth factor(bFGF) in the uterine leiomyoma and adjacent normal myometrium,and the relationship between in the them.By research the role of Cyr61 and bFGF in the pathogenesis of uterine leiomyoma.To provide a theretical basis for new conservative means to treat uterine leiomyoma.Method:(1)Culture human isolated uterine leiomyoma cells and smooth muscle cells,to identification the purity of the cells by the immunocytochemical method.(2)The cells for experiment were randomized assigned to three groups:A group:to determine the levels of Cyr61; B group:to determine the levels of bFGF;C group:the cells were treated with the bFGF,then were used to measure the level of cyr61,at 0h,0.5h,1h,2h,4h,8h,24h.(3)The method to determine the levels of Cyr61 and bFGF by Reverse transcription-polymerase chain reaction (RT-PCR).Results:1.labeling culture cells withα-SMA-actin,showed the 90%of culture cells areα-SMA-actin positive cells namely the uterine leiomyoma cells/smooth muscle cells.2.The expression of Cyr61 was observed in both the uterine leiomyoma cells and smooth muscle cells. Cyr61 level in uterine leiomyoma cells was significantly lower than in smooth muscle cells.(P＜0.001).The levels of cyr61 in the secretory phase and in the proliferative phase is no significant difference.(P＞0.05).3.The expression of bFGF was observed in both the uterine leiomyoma cells and smooth muscle cells.bFGF level in uterine leiomyoma cells was higher than in smooth muscle cells.(P＜0.05).4.The cells were treated with the bFGF,then were used to measure the level of cyr61,at Oh,0.5h,1h,2h,4h,8h,and 24h.In the smooth muscle cells,the levels of Cyr61 has a significantly increasing.Get the peak in 1 hour,then it is back down.At the 0.5h/1h/2h,the levels of Cyr61 has a significantly difference(P＜0.001).Others groups is no significant difference(P＞0.05).Paradoxically,in the uterine leiomyoma cells:in 24 hours, the levels of cyr61 is no significant difference.(P＞0.05).Conclusion:1.The expression of Cyr61 in uterine leiomyoma cells was significantly lower than in smooth muscle cells.2.bFGF as a important growth factor,bFGF level in uterine leiomyoma cells was higher than in smooth muscle cells.3.In the smooth muscle cells,the bFGF can up-regulation the Cyr61,but in the uterine leiomyoma,it can't.