Therapeutic Effect And Underlying Mechanisms Of IL-24 For Human Nude Mice Model Of Cervical Cancer

Objective To investigate the inhibition function and underlying mechanisms of IL-24 used alone or incombination with DDP on human cervical cancer nude mice model tumor growth and metastasis.Methods After the model of human cervical cancer xenografts was established in nude mice, 30 mice wererandomly divided into 5 groups: PBS (A group), empty vector plasmid (B group), IL-24 recombinant plasmid(C group), Half dose DDP (D group), DDP(E group), IL-24 recombinant plasmid + DDP (F group). All groupsof nude mice were respectively treated with injected 0.1mg PBS, 0.1mg empty vector plasmid (100 mg/L),0.1mg IL-24 recombinant plasmid (100 mg/L), 0.5ml DDP (2.5mg/kg), 0.5ml DDP (5mg/kg),IL-24recombinant plasmid 0.5ml DDP (2.5mg/kg) and IL-24 recombinant plasmid, into transplanted tumor withLipofectamine 2000 to interfere with the growth of transplanted tumor every three day, and DDP was injectedperitoneal. During the period of treatment, the size and volume of tumor were measured and the systemicconditions of the mice were observed. On the 15th day after treatment, the nude mice were sacrificed, andtumor tissues were collected and weighted to calculate the rate of tumor inhibition. Determination of IL-24 andVEGF-C expression in transplanted tumor by RT-PCR, and the expression of VEGF-C was detected withimmunohistochemical technology and western blot.Results After transfection IL-24 gene was successfully transfected into tumor cells and has been successfullyexpressed in tumor cells. To the end of the experiment, tumor volume in C, D ,E and F groups wassignificantly deflated than group A or group B (P<0.01); group F tumor volume was significantly lower thangroup C ,D orE (P<0.01). The inhibitory rate in the C, D, E and F groups was as follows: 29.74%、10.43%、16.95% and 38.37%, which was significantly less than that in the control group (P<0.01). After being inducedby IL-24, mRNA of VEGF-C was down regulated, and the results of immunohistochemical technology alsoshowed that the expression rate of VEGF-C in C and F groups decreased significantly compared to the controlgroup (P<0.01). Conclusion Combined application of IL-24 and DDP has stronger inhibition effect on tumorgrowth than single use of one drug, which has a synergic anticancer effect on transplanted cervical cancer innude mice. IL-24 can downregulate the expression of VEGF-C, which suppresses the growth and metastasizeof the cancer.