Effect Of FGF10Monoclonal Antibodies Against The Psoriasis-Like Model In Guinea Pigs

Psoriasis is characterized by epidermal hyperplasia with chronicinflammatory, and natural population incidence approximately1%-3%. T cells,dendritic cells and inflammatory mediators (such as: TNF-alpha, IL-8, IL-12,IL-22, IL-23) all involved in the pathogenesis of psoriasis,and playing animportant role in the induction of psoriasiskeratinocyte proliferation aspects. Infact, the proliferation of keratinocytes is the regulated by several cytokines,such as: epidermal growth factor, transcription growth factor a and fibroblastgrowth factors (FGFs). Among them, the keratinocyte growth factor (KGF) orFGF-7and the FGF-10or FGF10represent two important mediators ofkeratinocyte proliferation and differentiation. And the expression is increasedin psoriatic lesions.Objective：To investigate the effects of local topical treatment withfibroblast growth factor10（FGF10）monoclonal antibodies on psoriasiformlesions of guinea pigs. Provide a theoretical basis for the treatment of psoriasiswith FGF10monoclonal antibodies.Methods: Psoriasis-like animal models were established by treatingtopically ear back skin of guinea pigs with5%propranolol hydrochlorideemulsion. Set the blank group，model group, hydrocortisone butyrate treatmentgroup,FGF10monoclonal antibodies’ high （0.188mg/ml）、 medium（0.094mg/ml）、low（0.063mg/ml） dose treatment group respectively. Aftertwo weeks of treatment,whichever auricle and then observe the pathologicalchanges of psoriasis-like model under the microscope.Results: These indicators of each treatment group compared with modelgroup, all have significant difference (P <005). For the inflammatory cell count,high doses of FGF10antibody compared with blank group,no significant（P=0.443）,the remaining treatment group inflammatory cell count higher thanthe blank group and FGF10antibody high dose group, with significantdifference (P<0.05). FGF10antibody treatment group higher than model groupin epidermal thickness, with statistically significant (P <005).There was nosignificant between different concentrations of FGF10antibody group(P﹥005)in epidermal thickness.Conclusions: FGF10monoclonal antibodies against the psoriasis-likemodel of a significant treatment effect. In addition to the impact of keratinocyteproliferation, but also significantly inhibited the inflammatory response inpsoriasis model. High doses of FGF10antibody can make the numberinflammatory cells of model decreased to normal.