The Immunoregulation Of The "Yu Ping Ying Xing Fang" On Psoriasis With Damp-heat Syndrome: A Animal Stuy

Objective:1. Preliminary damp-heat syndrome psoriasis Syndrome combination ofanimal models of manufacturing method and evaluated.2. Observe the preventiveapplication the Yuping Yinxie square of damp-heat syndrome animal model of psoriasissymptoms, signs and immune function, Confirmed only by regulating the immune functionin patients to prevent or delay recurrence of psoriasis. Methods：1. Experimental guineapigs were randomly divided into three groups, Normal control group, the psoriasis groupand damp-heat syndrome psoriasis model. Damp-heat syndrome type psoriasis mademodule composite multi-factor modeling method, Give guinea pigs Feigan diet plus liquorand placed in a hot and humid outside environment in order to create the model ofdamp-heat syndrome, While propranolol The emulsion manufacturing guinea psoriasismodel,to simulate the damp-heat syndrome psoriasis disease and syndrome animal model;Signs and symptoms, lipids, rectal temperature by guinea pig model and BTE observationand detection of skin disease management changes,and the Evaluation Whether the animalmodel success.2. The experimental animals were randomly divided into four groups,normal control group, psoriasis, psoriasis made module of damp-heat syndrome andYuping Yinxie square group. Prevention group in made die at the same time theprophylactic use of jade screen silver dust party, the other three groups with theexperimental one are the same. After the building was carried out on the model of guineapigs skin symptoms, signs and behind the ears pathology observation and testing, at thesame time by means of flow cytometry instrument detection of peripheral blood Tlymphocyte subsets (the ratio of CD4+/CD8+) of the change, using the ELISA method todetect each group of experimental animals ratio of the IFN-γ/IL-4(i.e. the ratio of Th1/ Th2), evaluation of each animal cell immunity levels change.And by harvesting animalthymus gland, thymus index is calculated,then observe the changes of each buildinganimal immune organs. Results:1. Damp-heat syndrome type psoriasis guinea pigs at thesame time the typical symptoms and signs of damp-heat syndrome and psoriasis, bloodlipids, anal temperature level is significantly higher than blank group and pure psoriasis（P<0.01）, Behind the ears skin pathological grading Baker and inflammatory cellsinfiltrating the number is higher than the blank group and pure psoriasis （P<0.01）.2.After the prophylactic use of jade screen silver crumbs party, building animals comparedwith damp heat syndrome psoriatic group of guinea pigs, psoriasis symptoms appear slow,thymus index rise（P<0.05）, behind the ears skin pathological score and the number ofinflammatory cells infiltrating Baker is decreased obviously （P<0.05）, and the ratio ofTh1/Th2and CD4+/CD8+is also lower（P<0.05）. Conclusion:1. Damp heatsyndrome psoriatic animal models with both the pathological characteristic of psoriasis,and conform to the damp heat syndrome of TCM syndromes.2. The Yuping Yinxie only byadjusting the level of guinea pig immune organs and immune T lymphocytes to achieve theprevention or slowing psoriasis role.