The Experimental Study Of Hydrogen Peroxide On The Articular Cartilage Damage

Objective: pathological observation of the articular cartilage afterdifferent times of the hydrogen peroxide treatment, hydrogen peroxide with orwithout articular cartilage damage, and deal with the relationship between timeand the extent of damage of the articular cartilage. For deeper opening of thejoints involved in the clinical suspect that the use of anaerobic infection in thewound debridement and hydrogen peroxide to provide the experimental basis,to explore whether there is a reasonable point in time, either the effectiveprevention of bacterial infections. also can not articular cartilage damage, orjust to cause reversible damage. In order to avoid the articular cartilage damage,aggravated by the use of hydrogen peroxide to increase the incidence ofpostoperative traumatic arthritis, which cause unnecessary suffering to thepatient.Methods: China-Japan union hospital of jilin university tissue librariesprovide fresh knee specimens (select part of the specimen without surfacedamage). Preferred fixed, without any processing specimens as a blank control.Secondly, the samples were sharp cutting and randomly divided intoexperimental and control groups. According to the clinical and practical，control group and experimental groups were divided into group30seconds,60seconds,90seconds,180seconds,5minutes group,10minutes group, a totalof six groups. while the specimen is placed in the container, Add normal salineto the control group and hydrogen peroxide to the experimental group in thesame time.wait for30seconds,60seconds,90seconds,180seconds,5minutes,10minutes, take them off and at the same time put into the prior readyfixative.24hours later, cut down the cartilage from the complete specimens, remove the edge and irregular part, sacrificing part of the production size0.5x0.5cm two. Attention to protecting the cartilage surface, to prevent humaninjury in the production process. Other groups was done like this Finally,paraffin-embedded, HE staining, toluidine blue staining, immunohistochemicalstaining of slices produced. After production, the changes in the structure ofcartilage tissue were watched under the microscope.Results:1generally observed when the specimen were injected byhydrogen peroxide, due to the strong oxidation of hydrogen peroxide, andinstantly see a large number of bubbles generated. Then observed the color ofthe specimen we can see: the control group remained normal cartilage lightblue, the experimental group loss the normal color, The cartilage surface isslightly rough, and change significantly with immersion time long.2HEstaining In the control group changes，There is no significantly change. In30seconds,The experimental group only have a small number of cell vacuolesin the shallow surface of the outer layer, in90seconds the bubblegradually increasing, and the emergence of a hierarchical, more severe in180seconds, but in5minutes and10minutes, although the surface is good than180seconds reduced, but the change of the transition layer, indicating that thedamage to further deepen the band.3Toluidine blue staining the experimentalgroup and control group cartilage morphological changes consistent with HEstaining. Loss of dye level in the control group no significant changes, theexperimental group with the time of30seconds have a mild loss of dye,180seconds have a moderate loss of dye,10minutes have a severe loss of dye,there is no non-staining, These indicating that the cartilage matrix destruction isgradually increased with time increment.4Immunohistochemical observationthe experimental group and control group cartilage cell morphology changewas consistent with the other two staining methods, collagen type II staininghave no significant difference in the change.5Mankin score in each time period, the experimental group have a high score than in the control group, Inthe experimental group with time become long the score increased.There isstatistically significant mean.(P <0.01).Conclusion:1.cartilage was damage begin with30seconds.The damageaggravated with the time go on. At five minutes, the damage to the thebreakthrough cartilage surface with up to the transition zone, ballooningdegeneration of cartilage cells appear. Damage to the cartilage matrix with thetime go on spread to the deep and increase the degree of cartilage loss of dye.The impact of type II collagen there is no significant changes.2.In the clinicaldebridement should not use hydrogen peroxide on the articularcartilage.hydrogen peroxide in30seconds can not reach a good bactericidaleffect, but has caused damage.