Monitoring Of The Virus Antibodies During The Process Of SPF Non-human Primates Populations Establishment

Objective: Nonhuman primates are the closest animals on kinship compared withhuman, also with the high similarity on organizational structure, immunology,physiology and metabolism. In the recent years, following with the rapiddevelopment of the life sciences, the demands for experimental nonhumanprimates were increasing fastly. Expanding the nonhuman primate populations, gettingthe high quality, purity and stability non-human primates have become an urgentrequirement for the further development of the scientific research in China. Although itwill be very expensive, the SPF (specific pathogen-free) core group, once which waseatablished, can be used to provide high quality nonhuman primates forscientific research continuously. For the nonhuman primates may carry many kinds ofviruses, the monitoring of the viruses should be extremely important and will directlyaffects the establishment of the SPF core group.This research were conducted throughout the whole process of establishing theSPF core group in a big monkey breeding base in Jiangsu provience, focused on theCercopithecine herpesvirus1(BV), Simian the type D retrovirus(SRV), Simian immunodeficiency virus (SIV) and Simian T-lymphotropic virus1(STLV). A long-term, large-scale virus monitoring was designed in order to find aproper frequency to monitor the four virus, and thus improve the success rate of the SPFcore group population establishment.Methods: The Enzyme-linked Immunosorbent Assay (ELISA) and/or the DotImmunoassay (DIA) technology were employed to test the BV, STLV, SIV and SRV.The statistical software SPSS13.0and the Fisher exact test were employed to make thestatistical analysis of the follwing contents:(1) the comparison of BV, STLV, SIV andSRV levels on adults and juveniles cynomolgus or rhesus monkeys;(2) the omparisonof BV, SRV, SIV and STLV levels of the female and male juveniles of cynomolgus orrhesus monkeys;(3) the comparison of BV, SRV, SIV and STLV levels of the female and male adults of cynomolgus or rhesus monkeys;(4) the comparison of BV, SRV,SIVand STLV levels of the cynomolgus and rhesus juveniles;(5) the comparison of BV,SRV, SIV and STLV levels of the cynomolgus and rhesus adults.Results:(1) BV: For cynomolgus monkeys, the statistical analysis shows that thevirus infection rates in female juveniles and adults, female and male juveniles were nostatistic difference; the virus infection rate of the male juveniles and adults were1.1%and7.5%, which was statistic difference (P=0.031). And the virus infection rate ofmale adults (7.5%) were statistically higher than the female adults (2.2%)(P=0.009).For the rhesus monkeys, the virus infection rates in the male juveniles and adults,female and male juveniles, female and male adults, were no statistical differences; thevirus infection rates of the female adults (7.3%) was statistically higher than the maleadults (0.5%)(P=0.010). Compare the cynomolgus and rhesus, there were nosignificant difference in male juveniles, female adults, male adults; in female juveniles,the virus infection rate of cynomolgus (7.3%) was significantly higher than the rhesus (0)(P=0.002).(2) STLV: For cynomolgus and rhesus, there were no significant differenceof the virus infection rate between the female juveniles and adults, male juveniles andadults, female and male adults. Compared the cynomolgus and rhesus, there were nosignificant difference in female juveniles, male juveniles, female adults, male adults.(3)SIV: For cynomolgus, there were no significant differences of the virus infection rate infemale juveniles and adults, male juveniles and adults, female and male juveniles,female and male adults. For rhesus, there was no significant difference of the virusinfection rate in female juveniles and adults, female and male juveniles, female andmale adults; the virus infection rate of male adults (6.3%) was statistically higher thanthe female adults (0.7%)(P=0.028). Compared the cynomolgus and rhesus, they wereno significant difference in female juveniles, male juveniles, female adults and maleadults.(4) SRV: For cynomolgus, there was no significant difference of the virusinfection rate in female juveniles and adults, male juveniles and adults, female and malejuveniles, female and male adults. For rhesus, there were no significant difference of thevirus infection rate in female juveniles and adults, female and male juveniles, femaleand male adults; the virus infection rate of male adults (1.6%) was statistically higherthan the female adults (0.7%)(P=0.028). Compared the cynomolgus and rhesus, theywere no significant difference in female juveniles, male juveniles, female adults andmale adults.(5) Effective frequency of virus detection: the virus was detected in the same cynomolgus monkeys separated by3months, the infection rate of animalstransferred to virus positive were calculated as follows: BV (3/58), STLV (0/55) andSRV (0/55). However, if separated by1month, the virus (BV/STLV/SRV) infection rateof animals transferred to positive were0/93.(6) Comparison of Colloidal Gold Methodand ELISA or DIA: the compliance of colloidal gold method and ELISA on BV andSTLV were88.9%and81.8%; the compliance of colloidal gold method and DIA onSRV was77.3%.Conclusion: During the process of establishing SPF cynomolgus and rhesus coregroup, the following conclusions were drawed based on the current feeding andmanagement mode, virus detection technology and the frequency of testing:(1) thecynomolgus monkey and rhesus monky infecting the BV, SRV, SIV and STLV continueto be maintained at a low virus level;(2) the infection rates of BV in the cynomolgusand rhesus monky were increased with age;(3) the infection rate of SIV on themale rhesus monkey was increased with age;(4) under the current management mode,the frequency of monitoring the STLV, SIV and SRV were suitable on each threemonths, while the frequency for BV should be one month.