The Experimental Study Of The Efficacy Active Selection In Extracts Of Xiongma Decoction

Objective:Using the flash type extraction method, get the extract of Petroleum ether, Dichloromethane, Ethyl acetate and Butanol from Xiongma decoction respectively. And then study the Xiongma decoction and its extracts on migraine model (nitroglycerin type, reserpine low5-HT type) in behavioral symptoms, weight, and coagulation time, blood index, etc. preliminarily screen the Xiongma decoction’s effective parts for migraines, and research its effective parts on the anti-inflammatory and analgesic experiments.Methods:Using different polarity organic solvent to extract Xiongma decoction, with time, number, solvent quantity for index, to reduce pressure concentration, and flick dry solvent, get the extract of Petroleum ether, Dichloromethane, Ethyl acetate and Butanol of Xiongma decoction; With NGT model rats for research object, and with the number of scratching and climbing, the plasma of5-HT and NO for observation index, To study the influence of Xiongma decoction and its extractson in the migrane rats model by nitroglycerin; With reserpine low5-HT model mouth for research object, and with weight, coagulation time and the plasma of5-HT and NO for observation index, To study the influence of Xiongma decoction and its extractson in the mouth model by reserpine; With hot plate experiment to study the Analgesic action of Xiongma decoction and its effective parts; With the experiment of inflammation model by acetic acid to study the anti-inflammatory of Xiongma decoction and its effective parts.Results:①To get the extract of Petroleum ether(10.8), Dichloromethane(8.1), Ethyl acetate(3.4) and Butanol(1.7) of Xiongma decoction from original medicine yinpian(372g).iongma decoction and tis Butanol extraction might have inhibition of the number of scratching in the migrane rats model by nitroglycerin; Xiongma decoction might have inhibition of the number of model rats’climbing(P <0.05).iongma decoction and its Butanol extraction could reduce the model rats’plasma of5-HT and NO significantly(P<0.01).iongma decoction and its its Butanol extraction could increase weight and prevent excessive decrease in5-HT(P<0.01); Xiongma decoction and its extracts of Ethyl acetate and Butanol could prolong coagulation time and improve the content of NO(P<0.01).iongma decoction and its extracts of Ethyl acetate and Butanol could have analgesic action.iongma decoction and its Butanol extraction could have anti-inflammatory action.