Proteomic Analysis Of Autologous Bone Marrow Derived Neural Stem Cell Transplantaiton For Treatment Of Central Nervous System Injury

Background: War wounds, traffic accidents, natural disasters, and cerebralvascular accident can cause a large number of nervous system damage, centralnervous system damage and poor prognosis, often leaving patients withsymptoms that are difficult to restore, such as paraplegia, hemiplegia, aphasia,vision disturbances and so on. Cause great difficulties in the lives of patients, Ibring a heavy burden to the patient’s family. The treatment of central nervoussystem injury is a major difficulty in neurosurgery, the effect of conventionaldrugs and physical therapy is not satisfactory. Bone marrow derived neural stemcell transplantation is the treatment of central nervous system damage in a newway of thinking, and has broad prospects, but for now, the early efficacy ofneural stem cell transplantation for treatment of central nervous system injury isnot significant, is not conducive to clinical observation. Proteomics technologydevelopment to solve this problem a new method, we used proteomic analysis of the patient group and normal control group as well as neural stem celltransplantation in patients with cerebrospinal fluid proteomics, and further studynervous system injury in the cerebrospinal fluid of patients with proteomics, andexpectations in order to evaluate the early efficacy of neural stem celltransplantationObjective:1. Preliminary study of proteomics and nerve function.2. Study theproteomics of cerebrospinal fluid of the central nervous system injury.3..Application of proteomic methods to analyze the efficacy of stem celltransplantation in the central nervous system damage.Materials and Methods: A. Normal adult collected from40cases (group1, n=40,114marked), central nervous system damage in40patients (group2, n=30,115marked), a week after surgery in patients with cerebrospinal fluid (group3, n=30,116marked), as well as neural stem cell culture supernatant5ml (group4,117marked); B.Normal adult collected from40cases (group1, n=30,114marked), sham operation group of40cases of preoperative and postoperative(group5, n=40,114,115marked), the same amount of mixing within the groupto remove high abundant proteins using high performance liquid column, thesample after iTRAQ reagents markers using two-dimensional chromatographicseparation by tandem mass spectrometry identificationand relative quantificationof proteins retrieved from the protein Data Bank.Results: Mass spectrometry to get the peptide fragment spectral library search for identification of the protein the59neural stem13before the celltransplantation group and normal control group differences in the protein, and5proteins were highly expressed, eight showed low expression.13proteins,seven proteins in the neural stem cell transplantation group and normal controlgroup showed no difference; sham operation, preoperative expression of thebasic with the neural stem cell transplantation preoperative group, no significantdifference between sham operation group, preoperative and postoperative.Conclusion: Proteins play a very important role in the nervous system, thetransformation process, analysis of the changes of various proteins in the CSFproteomics methods can be effective, provides a very important tool for us tounderstand the physiological and pathological changes of the nervous system,can be found in the nervous system to micro, qualitative and semi-quantitativechanges, to provide a basis for early diagnosis and efficacy evaluation. Throughour research found that more positive effect of autologous bone marrow derivedneural stem cell transplantation for treatment of central nervous system damage,injury treatment of central nervous system a very promising treatment.