| Objective To evaluate the effects of minocycline gel made in the research group in the treatment of rats’ experimental periodontitis. To evaluate and compare the controlled-release characteristic and clinical effect of minocycline gel and periocline as an adjunctive therapy to scaling and root planning(SRP) in periodontitis.Methods Experimental periodontitis was induced by ligature technique and Porphyromonasgingivalis(P.g) inoculation in male Sprague-Dawley rats, then the rats were fed with100g/L high sucrose drinking water during7weeks.16adult male Sprague-Dawley rats were divided into4groups randomly:①Minocycline gel group: rats with periodontitis treated by minocycline gel,②PBS group:rats with periodontitis treated by PBS,③Model group:periodontitis rats without any treatment and④the blank control group. We recorded the weight of rats, mobility (MOB) of periodontitis, probing depth (PD) and sulcus bleeding index (SBI). After four-week-treatment, the tissues around the test site were excised, samples were embedded and fixed, then the histological assessment were carried out by HE staining to evaluate inhibition effects of minocycline gel on periodontal tissue inflammation. In clinical experiment,22tooth in11patients with moderate to advanced periodontitis were randomly divided into two groups:SRP plus minocycline gel and SRP plus periocline. All clinical parameters including SBI, PD, MOB and the quantity of gingival crevicular fluid were recorded at base line and4weeks after treatment. The drug concentration in periodontal pocket was measured by collecting gingival crevicular fluid after drug using.Results During the animal study, there were no obvious changes in the weight of rats in any groups and no symptom of toxicity were observed. After four-week-treatment, minocycline gel group could significantly improved the periodontal clinical symptoms and lighten the periodontal inflammation. The gingiva returned to normal gradually and the mobility decreased. Compared with PBS group, minocycline gel seemed more efficiently improved in PD and SBI of the rats, and there was significantly difference between two groups (P<0.05); There was significantly difference of periodontal clinical paraments between Minocycline gel and the blank control group (P<0.05) after treatment. The pathology examination of HE staining also indicated that the test sites which were treated with minocycline gel had a certainly control of gingival inflammation, and the tissue could be repaired. In clinical experiment, all clinical parameters in two groups had no significant difference at4weeks after treatment; Two drugs released slowly after using in periodontal pocket and the effective drug concentration could be maintained for one week. The concentration of minocycline gel was3.334mg/1at the first day and1.857mg/1at the seventh day, while the concentration of periocline was3.341mg/1to0.714mg/l.Conclusion Our study showed that minocycline gel is a kind of effectively and safe agent without toxicity, it can effectively improve the periodontal tissue inflammation in rats. It releases slowly in periodontal pocket and has a similar clinical effect with periocline in the adjunctive therapy of Chronic Periodontitis. It possesses clinical application value. |
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