Association Between VDR Gene Polymorphisms And Diabetic Retinopathy

Objective:To study the relevant risk factors for2diabetic retinopathy(DR).In addition,we alsoinvestigate the polymorphism of Vitamin D receptor (VDR)gene in Chinese Hanpopulation Anhui region,and the association of which with Diabetic retinopathy (DR)susceptibility,Methods:Three hundreds and seventy nine cases of T2DM patients with fretinal, the bloodpressure, glycated hemoglobin(HbA1C), triglycerides(TG), cholesterol(CH), highdensity lipoprotein (HDL), low density lipoprotein(LDL), serum uric acid (UA), serumcreatinine (Cr),blood urea nitrogen(BUN), alanine aminotransferase(ALT), body massindex (BMI),aspartate aminotransferase(AST) and alkaline phosphatase (ALP) wereanalyzed. Three hundreds and ten were devided into three groups: type2diabetic(T2DM) group,110cases(male52,female58), control group,116cases (male59,female57) and Diabetic retinopathy (DR) group,94cases(male44,female50)Genomic DNAwere extracted from peripheral white blood cell. The polymorphism of Vitamin Dreceptor (VDR) gene at BsmI, FokI, ApaI were determined by polymerase chainreaction and Restriction fragment length polymorphism analysis,via agarose gelelectrophoresis to read allele and genotype. The frequencies distrubition of allele andgenotype between DR group and T2DM group, DR group and normal control group,T2DM group and normal control group were compared by chi square applying SPSS16.0statistical software. Results:(1)The overall prevalence of DR in patients with T2DM was31.13%,non-proliferativeDR (NPDR) accounted for24.01%, proliferative DR (PDR) accounted for7.12%. Inhospitalized patients,DR overall prevalence was32.2%,NPDR accounted for23.26%and PDR accounted for8.91%;HbA1C was no significant correlation with DR;Thecourse of DR was longer than that of patients with T2DM (P<0.05),The diseaseduration of PDR patients was significantly longer than NPDR patients (P<0.05); Levelsof systolic and diastolic biood pressure in DR patients were higher than those in T2DMpatients (P<0.05), levels of which in PDR patients were higher than in NPDR patients(P<0.05).In outpatients, DR the total prevalence of was28.81%,NPDR accounted for25.4%,PDR accounted for3.39%,the course disease,blood pressure was no significantcorrelation with DR,the recent HbA1C of DR patients was higher than T2DM patients(P <0.05),the recent HbA1C of PDR patients was higher than NPDR patients (P<0.05).(2) VDR FokI The f allele frequeney (61.70%) of FokI locus in DR group wassignifieantly higher than the frequeney (40.45%) in T2DM group(P<0.05,OR=2.284)；the frequeney of ff genotype(32.98%) of FokI locus in DR group was signifieantlyhigher than the frequeney(10.91%) in T2DM group(P<0.05，OR=4.019). The f allelefrequeney (61.70%) of FokI locus in DR group was signifieantly higher than thefrequeney (40.52%) in normal contro group(P<0.05,OR=2.365)；the frequeney of ffgenotype(32.98%) of FokI locus in DR group was signifieantly higher than thefrequeney(15.52%) in normal contro group(P<0.05,OR=2.679).There were nosignificant difference(p>0.05) of the frequencies distrubition of allele and genotype ofFokI locus between T2DM group and normal control group.(3) VDR BsmI The Ballele frequeney (17.73%) of BsmI locus in T2DM group was signifieantly higher thanthe frequeney (9.48%) in normal control group(P<0.05,OR=2.068);the frequeney ofBB+Bb genotype(28.18%) of BsmI locus in T2DM group was signifieantly higher thanthe frequeney(17.24%) in normal control group(P<0.05,OR=1.884). The B allele frequeney (19.68%) of BsmI locus in DR group was signifieantly higher than thefrequeney (9.48%) in normal contro group(P<0.05,OR=2.47);the frequeney of BB+Bbgenotype(33.04%) of BsmI locus in DR group was signifieantly higher than thefrequeney(17.24%) in normal contro group(P<0.05,OR=2.924).There were nosignificant difference(p>0.05) of the frequencies distrubition of allele and genotype ofBsmI locus between T2DM group and DR group.(4). There were no significantdifference(p>0.05) of the frequencies distrubition of allele and genotype of ApaI locusbetween T2DM group and DR group,T2DM and normal contro group,DR group andnormal contro group.Conclusion:(1)For long-term adherence better outpatients, there are no correlation DR patients withduration of disease,blood pressure,CH,TG,LDL,HDL,Cr,UA,BUN,ALT,AST and ALP;DR are associated with HbA1C.For hospitalized patients,DR are related with durationof blood pressure; Disease and blood pressure are important risk factors for DR.pressure; Disease and blood pressure are important risk factors for DR.(2)There areVDR gene FokI;BsmI;ApaI Polymorphisms in Chinese Han population Anhui region.(3)There is association of VDR gene FokI polymorphism with DR susceptibility inChinese Han population Anhui region. There are no association between VDR geneFokI polymorphisms and T2DM susceptibility in Chinese Han population Anhui region.(4) There is association of VDR gene BsmI polymorphism with T2DM susceptibility inChinese Han population Anhui region. There are no association between VDR geneBsmI polymorphisms and DR susceptibility in Chinese Hanpopulation Anhuiregion.(5)There are no association between VDR gene ApaI polymorphisms and DRsusceptibility in Chinese Hanpopulation Anhuiregion. There are no association betweenVDR gene ApaI polymorphisms and T2DM susceptibility in Chinese HanpopulationAnhuiregion.