Preparation And Experiment In Vitro Of Folate-chitosan Magnetic Nanoparticles

Objective:To prepare folate chitosan magnetic nanoparticles and optimize the preparation method. To evaluate the toxicity of folate chitosan magnetic nanoparticles in human breast cancer cells(MCF-7), in which folate receptors highly express, and the uptaken by MCF-7based on vitro experiments, and to explore the tumor targeting of folate chitosan magnetic nanoparticles.Methods:Firstly, the magnetic nanoparticles were synthesized by coprecipitation and the surface of magnetic nanoparticles modified with chitosan and folate. Secondly, the cell growth rate of MCF-7was detected through the MTT method by magnetic nanoparticles, chitosan magnetic nanoparticles and folate chitosan magnetic nanoparticles. Then, FITC was used to mark the chitosan magnetic nanoparticles and folate chitosan magnetic nanoparticles and cultivated with MCF-7a few hours later. Finally, the expression of two marked particle in MCF-7cell with fluorescence microscope was observed.Results:The particle size of magnetic nanoparticles was under lOnm. And the average particle size of folate chitosan magnetic nanoparticles was45.12nm. The particles were good dispersion and stable so that it met the requirement of medicine to target cancer cells. The result of MTT experiment showed that the cell growth rate of folate chitosan magnetic nanoparticles to MCF-7was lower than magnetic nanoparticles, but higher than chitosan magnetic nanoparticles (p<0.05). Under the fluorescence microscope we could see that MCF-7uptaked folate chitosan magnetic nanoparticles much more than uptaked chitosan magnetic nanoparticles.Conclusions:The folate chitosan magnetic nanoparticles could target the cancer cells, with low toxicity and high targeting property of folate receptors.